毛乳头细胞微囊诱导大鼠耳毛囊再生

目的:应用人头皮毛乳头细胞APA(alginate-polylysine-alginate,海藻酸钠-多聚赖氨酸-海藻酸钠)微囊诱导大鼠耳部毛囊再生。方法:以APA微囊包裹体外分离培养的人头皮毛乳头细胞;将毛乳头细胞微囊移植至大鼠耳部皮下,定时行组织学检查。结果:毛乳头细胞微囊移植3周后,组织学检查显示:移植部位皮下形成有密集的同心圆状毛囊结构形成,其数量、形态、分化程度等与正常鼠耳毛囊明显不同。结论:微囊化毛乳头细胞具备诱导大鼠耳部毛囊再生的功能。

Induction of Hair Follide Regeneration in Rat Ear by Dermal Papilla Cell Mieroeapsules

Objective To induce the hair follicle regeneration in rat ear by microencapsulated dermal papillae cells(DPs).Methods The DPs were encapsulated with alginate-polylysine-alginate by a high-voltage electric field droplet generator.The DP microcapsules were xenotransplanted into the rat ears.Histological examination was made by microscopy.Results Fully developed hair follicles could be easily identified in the skin of implanted site following xenotransplantation of reconstructed DP,which were different from the normal hair follicle of rat ear in configuration,number,size and differentiation degree.Conclusions The microencapsulated DPs retains the physiological function to induce the follicle regeneration.