非诺贝特和阿托伐他汀对ApoE基因敲除小鼠外周血单核细胞TLR4表达的影响

目的 探讨非诺贝特和阿托伐他汀对动脉粥样硬化(atherosclerosis, AS)模型小鼠单核细胞Toll样受体4(Toll-like receptor 4, TLR4)表达的影响.方法 apoE基因缺陷(apoE deficient,apoE-/-)小鼠被随机分为3组,分别为阿托伐他汀干预组、非诺贝特干预组、模型组,每组10只.前两组分别给予阿托伐他汀10 mg·kg-1·d-1和非诺贝特100 mg·kg-1·d-1,对照组给予等量蒸馏水灌胃.8周后,测定血清低密度脂蛋白(LDL)、甘油三酯(TG)、总胆固醇(TC)水平,并应用流式细胞仪检测各组小鼠外周血单核细胞表面TLR4的表达.结果 各组小鼠血脂水平无统计学差异.与单纯高脂餐喂养组相比,非诺贝特干预组小鼠TLR4表达明显降低(P=0.02);阿托伐他汀干预组TLR4表达轻度升高,但无统计学意义.结论 非诺贝特可降低TLR4表达,可能参与AS进程中介导的天然免疫反应.

Effects of fenofibrate and atorvastatin on Toll-like receptor (TLR) 4 expression in ApoE-deficient mice

Objective To observe the effects of fenofibrate and atorvastatin on Toll-like receptor(TLR) 4 expression in ApoE-deficient mice.Methods ApoE-deficient mice were divided into 3 groups which received high-fat diet(HF) alone and were treated with fenofibrate(HF+F) or atorvastatin(HF+A) for eight weeks,respectively.Serum level of total low-density lipoprotein,cholesterol,triglyceride,total cholesterol was detected after 8 weeks,respectively.Results No significant difference of serum level of total low-density l...