| PCR扩增石蜡包埋小鼠组织p53基因突变热点片段 |
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| 引用本文： | 戴文涛,袁晶,陈学敏.PCR扩增石蜡包埋小鼠组织p53基因突变热点片段[J].中国公共卫生,2001,17(1):24-26. |
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| 作者姓名： | 戴文涛 袁晶 陈学敏 |
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| 作者单位： | 1. 现在广东省江门市卫生防疫站 529050
2. 同济医科大学环境卫生学教研室武汉 430030 |
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| 摘 要： | 目的 扩增石蜡包埋小鼠组织p53基因第5、7外显子。方法 用3种方法提取DNA，比较提取效果，用聚合酶链式反应（PCR）扩增相应片段。结果 用二甲苯脱蜡，蛋白酶K消化120小时可获得较好的DNA提取物。结论 用该提取物为模板扩增小鼠p53基因突变热点片段成功率高。
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| 关 键 词： | 聚合酶链式反应 石蜡包埋 小鼠 P53基因 |
| 文章编号： | 1001-0580(2001)01-0024-03 |
| 修稿时间： | 2000年4月4日 |
Amplifing p53 Gene Hot
Mutation Segment of Pareffin Embedded Mouse Tissue with PCR |
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| DAI Wen tao,et al..Amplifing p53 Gene Hot
Mutation Segment of Pareffin Embedded Mouse Tissue with PCR[J].Chinese Journal of Public Health,2001,17(1):24-26. |
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| Authors: | DAI Wen tao |
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| Institution: | Wuhan 430030 |
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| Abstract: | Objective To amplify the p53 gene exon 5 and exon 7 of the paraffin embedded mouse tissues.Methods The DNA was extracted with three methods and the extraction effects were compared with each other,PCR was used to amplify those exons.Results Better DNA of the tissues treated with xylene and digested 120 hours with proteinase K was obtainable.Conclusions A high successful rate can be acquired using these DNA as templates to amplify the mouse p 53 gene hot mutation segments. |
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| Keywords: | polymerase chain reaction paraffin embedded mouse p 53 ge |
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