| IL-4基因治疗脑胶质瘤的实验研究 |
| |
| 引用本文： | 幸兵,任祖渊,苏长保. IL-4基因治疗脑胶质瘤的实验研究[J]. 中华肿瘤杂志, 1999, 0(1): 13-15 |
| |
| 作者姓名： | 幸兵 任祖渊 苏长保 |
| |
| 作者单位： | 中国医学科学院中国协和医科大学北京协和医院神经外科 |
| |
| 基金项目： | 高等学校博士学科点专项科研基金 |
| |
| 摘 要： | 目的 探索一条细胞因子ＩＬ－４基因治疗脑胶质瘤的新途径。方法 用脂质体转染的方法，以重组逆转录病毒做载本，将目的基因鼠ＩＬ－４基因转入包装细胞和大鼠Ｃ６胶质瘤细胞，通过Ｇ４１８抗性筛选，得到分泌ＩＬ－４的细胞株Ψ２ ＩＬ－４和Ｃ６ＩＬ－４。Ｅｌｉｓａ法检测病毒上清中ＩＬ－的含量，通过Ｓｏｕｔｈｅｒｎ ｂｏｌｔ 和Ｄｏｔ ｂｌｏｔ证明ＩＬ－４基因的整合和表达。体外观察并比较了野生型和转基因瘤细胞的细
|
| 关 键 词： | 脑肿瘤 神经胶质瘤 白细胞介素4 基因治疗 |
Experimental study of interleukin 4 gene therapy for glioma |
| |
| XING Bing,REN Zuyuan,SU Changbao,et al.. Experimental study of interleukin 4 gene therapy for glioma[J]. Chinese Journal of Oncology, 1999, 0(1): 13-15 |
| |
| Authors: | XING Bing REN Zuyuan SU Changbao et al. |
| |
| Affiliation: | Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730. |
| |
| Abstract: | OBJECTIVE: To study IL-4 gene therapy for glioma. METHODS: Using recombinant retrovirus vector, mIL-4 was transfected into psi 2 packaging cells and rat C6 glioma cells, respectively by lipofectamine. The IL-4 secretting cell clones psi 2 IL-4 and C6IL-4 were studied in vitro and in vivo. RESULTS: Apart from a decrease in cloning efficiency, no difference was observed between the transfected and the wild type glioma cells in proliferation and cell cycle in vitro. Tumorigenicity of subcutaneously and intracranially inoculated C6IL-4 was reduced. Tumor growth could be suppressed by perineoplastic inoculation of psi 2 IL-4 cells. CONCLUSION: IL-4 gene-modified C6 glioma has decreased tumorigenicity. Local production of IL-4 by psi 2 IL-4 cells can lead to regression of established glioma. |
| |
| Keywords: | Brain neoplasms/Therapy Glioma/Therapy Interleukin 4 Retrovirus Gene therapy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
