|
|||||
|
|
| 9-氯甲酸芴甲酯柱前衍生高效液相色谱法测定奶粉中的牛磺酸 | |
| 引用本文： | 王晓莺,孙成均,张立实. 9-氯甲酸芴甲酯柱前衍生高效液相色谱法测定奶粉中的牛磺酸[J]. 环境与职业医学, 2009, 26(3): 301-304 |
| 作者姓名： | 王晓莺 孙成均 张立实 |
| 作者单位： | 苏州卫生职业技术学院检验药学系;四川大学华西公共卫生学院; |
| 摘 要： | [目的]建立一种简便、快速测定奶粉中牛磺酸的高效液相色谱测定方法。[方法]用纯甲醇沉淀奶粉样品中的蛋白后，以9-氯甲酸芴甲酯（FMOC）为柱前衍生剂，与奶粉中的牛磺酸发生衍生化反应后，反相高效液相色谱法测定含量。以甲醇-0．025mol／L磷酸盐缓冲溶液为流动相（pH=8．0），C18柱为分离柱，二极管阵列检测器（265nm）检测。[结果]本法相对标准偏差为3．38％，加标回收率范围为82．8％-96．7％，检出限为46ng。[结论]本方法具有快速、简单、准确度高的特点，适合于奶粉中牛磺酸的测定。 |
| 关 键 词： | 牛磺酸 高效液相色谱法 柱前衍生 奶粉 |
Determination of Taurine in Milk Powder by High Performance Liquid Chromatography after Derivatization with 9-Fluolenylmeghyl Chloroformate |
|
| WANG Xiao-ying,SUN Cheng-jun,ZHANG Li-shi. Determination of Taurine in Milk Powder by High Performance Liquid Chromatography after Derivatization with 9-Fluolenylmeghyl Chloroformate[J]. Journal of Environmental & Occupational Medicine, 2009, 26(3): 301-304 | |
| Authors: | WANG Xiao-ying SUN Cheng-jun ZHANG Li-shi |
| Affiliation: | 21.Laboratory Medicine and Pharmacy Department of Suzhou Health College;Suzhou;Jiangsu 215009;China;2.West China Public Health College of Sichuan University;Chengdu;Sichuan 610044;China |
| Abstract: | [Objective] To establish a high performance liquid chromatographic(HPLC)method for the determination of taurine in milk powder.[Methods] After deposited protein with methanol and derivatized with 9-fluolenylmeghyl chloroformate,tarine in milk powder was separated and quantified by HPLC.A mobile phase of methanol-0.025 mol/L phosphate buffer(pH=8.0),an analytical column of C18 and ultraviolet detector with wavelength on 265 nm were used in the study.[Results] The recovery ranged from 82.8%-96.7%,relative sta... |
| Keywords: | taurine HPLC derivatization milk powder |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |