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| 生物质谱技术及其在核酸领域的应用 | |
| 引用本文： | 杨莹莹,郑萍,叶利明. 生物质谱技术及其在核酸领域的应用[J]. 药物分析杂志, 2008, 28(4): 642-646 |
| 作者姓名： | 杨莹莹 郑萍 叶利明 |
| 作者单位： | 四川大学华西药学院，成都市食品药品检验所 |
| 摘 要： | 生物质谱技术具有准确、快速、灵敏度高等特点,因此在生物大分子领域得到了广泛应用。本文介绍了电喷雾电离和基质辅助激光解吸/电离质谱技术的基本原理,并综述了生物质谱在核昔酸的鉴定与定量分析、单核苷酸多态性分型以及寡聚核苷酸片断序列测定中的应用。 |
| 关 键 词： | 生物质谱 电喷雾电离(ESI) 基质辅助激光解吸/电离(MALDI) 核酸 |
Biological mass spectrometry and its application in the field of nucleic acid |
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| YANG Ying-ying ZHENG Ping YE Li-ming. Biological mass spectrometry and its application in the field of nucleic acid[J]. Chinese Journal of Pharmaceutical Analysis, 2008, 28(4): 642-646 | |
| Authors: | YANG Ying-ying ZHENG Ping YE Li-ming |
| Affiliation: | YANG Ying-ying~1 ZHENG Ping~2 YE Li-ming~ |
| Abstract: | Objective:The advantage of biological mass spectrometry is accuracy,high speed,high sensitivity,and it is widely applied in biomacromolecule domain.The principle of electrospray ionization-mass spectrometry (ESI-MS) and matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) is described in this text.Simultane- ously,its application in single nucleotide polymorphisms,characterization and quantification of nucleotide,and se- quencing of oligonucleotide is reviewed. |
| Keywords: | biological mass spectrometry electrospray ionization matrix assisted-laser desorption/ionization nucleic acid |
| 本文献已被 CNKI 维普 等数据库收录！ | |