| 不同来源HSP70的氨基酸组成分析 |
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| 引用本文： | 邬堂春 吴扬. 不同来源HSP70的氨基酸组成分析[J]. 中华劳动卫生职业病杂志, 1995, 13(3): 147-148 |
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| 作者姓名： | 邬堂春 吴扬 |
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| 作者单位： | 武汉同济医科大学职业医学研究所 |
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| 基金项目： | 卫生部科研基金和国家自然科学基金 |
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| 摘 要： | 使用ＤＥＡＥ－５２离子交换层析和ＡＴＰ琼脂糖亲和层析方法，从人体肿瘤细胞株，大鼠肝脏和心脏、家兔和小鼠肝脏组织中纯化了主要的热应激蛋白－ＨＳＰ７０，用氨基酸自动分析仪分析它的氨基酸组成。
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| 关 键 词： | 热应激蛋白70 氨基酸组分 劳动卫生学 |
ANALYSIS OF AMINO ACID COMPOSITION OF PURIFIED HSP70 OF SEVERAL ORIGINS |
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| Wu Tangchun,Wu Yang,Zhang Wei,et al Institute of Occupational Medicine,Tongji Medical Uniuersity,Wuhan. ANALYSIS OF AMINO ACID COMPOSITION OF PURIFIED HSP70 OF SEVERAL ORIGINS[J]. Chinese journal of industrial hygiene and occupational diseases, 1995, 13(3): 147-148 |
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| Authors: | Wu Tangchun Wu Yang Zhang Wei et al Institute of Occupational Medicine Tongji Medical Uniuersity Wuhan |
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| Affiliation: | Wu Tangchun,Wu Yang,Zhang Wei,et al Institute of Occupational Medicine,Tongji Medical Uniuersity,Wuhan 430030 |
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| Abstract: | The major heat stress protein HSP7o was purified from human leudaemia cancer cell line K562, rabbit liver,rat liver, ratheart and mouse liver with two-step procedures of DEAE-52 ion exchange chromatography and the affinity chromatography onATP-agaroea and their amino acid composition was detemined with Amino Acid Analyser. The result showed that the first threericliest amino acids in HSP70 of all origins tested were Glu Glu and Asp.except those of rat heart(Gly.Phe and Glu).Lys, Phe,Val,Leu and Ala were very ricb in HsP70 of all origins. The result proviued the referenced data for exploring the application ofHSP70 in labour hygiene and occupational diseases. |
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| Keywords: | HSP7o Amino acid conposition |
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