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| 带TrpC启动子的质粒pYG715／Vgb对顶头孢霉的转化 | |
| 引用本文： | 郑荣 朱春宝. 带TrpC启动子的质粒pYG715／Vgb对顶头孢霉的转化[J]. 中国抗生素杂志, 1999, 24(4): 269-272 |
| 作者姓名： | 郑荣 朱春宝 |
| 作者单位： | 上海医药工业研究院 |
| 摘 要： | 分别以来自于构巢曲霉和酿酒酵母的启动子ＴｒｐＣ和ＴＥＦ１来构建质粒ｐＹＧ７１５／ＴｒｐＣ和ｐＺＲ３３３并转化头孢菌素Ｃ工业生产菌种顶头孢霉，结果发现只有ｐＹＧ７１５／ＴｒｐＣ产生转化子。通过Ｓｏｕｔｈｅｒｎ杂交证明质粒整合进顶头孢霉的染色体中 |
| 关 键 词： | 顶头孢霉；启动子；质粒 |
Transformation of plasmid pYG715/Vgb bearing TrpC promoter in Cephalosporium acremonium |
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| Zheng Rong,Zhu Chunbao,Zhao Wenjie,Zhu Baoquan. Transformation of plasmid pYG715/Vgb bearing TrpC promoter in Cephalosporium acremonium[J]. Chinese Journal of Antibiotics, 1999, 24(4): 269-272 | |
| Authors: | Zheng Rong Zhu Chunbao Zhao Wenjie Zhu Baoquan |
| Abstract: | Cephalosporium acremonium , the producer of cephalosporin C, was transformed with plasmid pYG715/Vgb containing the both of a phleomycin resistance gene and a Vitreoscilla hemoglobin gene, which were cloned downstream of the TrpC gene promoter from Aspergillus nidulans . Analysis of Southern hybridization demonstrated the integration of the transformed DNA into the chromosomal DNA of Cephalosporium acremonium . |
| Keywords: | Cephalosporium acremonium Promoter Plasmid |
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