扩张型心肌病患者CD4~+CD25~+Foxp3~+ T细胞的检测及意义

目的:探讨扩张型心肌病(DCM)患者外周血CD4+CD25+Foxp3+T细胞的水平及意义。方法:采用流式细胞术检测DCM患者30例及健康对照组20例外周血CD4+CD25+T细胞和CD4+CD25+Foxp3+T细胞的比例。结果:DCM患者外周血CD4+CD25+T细胞占CD4+T细胞的比例为(8.53±1.64)%,显著低于健康对照组的(11.4±2.17)%,P0.01;DCM患者CD4+CD25+Foxp3+T细胞占CD4+T细胞比例为(0.99±0.54)%,显著低于健康对照组的(1.55±0.55)%,P0.01;且DCM患者心功能越差,CD4+CD25+Foxp3+T细胞占CD4+T细胞的比例越低。结论:DCM患者调节性T细胞比例的减少,可能打破了自身免疫耐受,发生了针对心肌抗原的自身免疫反应,参与了DCM的发病。

Significance of the change of CD4~+CD25~+ Foxp3~+ T cells in patients with dilated cardiomyopathy

Objective:To investigate the expression and significance of CD4~+ CD25~+ Foxp3~+ T cells in peripheral blood of patients with dilated cardiomyopathy (DCM). Method:The frequencies of CD4~+ CD25~+ T cells and CD4~+CD25~+Foxp3~+ T cells were detected in 30 patients with DCM (DCM group) and 20 healthy donors (control group) by flow cytometry. Result:The frequency of CD4~+ CD25~+ T cells ([8.53±1.64]%) was found to be significantly lower in patients with DCM than those of the control group([11.4±2.17]%) ,P<0.01. The frequency of CD4~+ CD25~+ Foxp3~+ T cells ([0.99±0.54]%)was found to be significantly lower in patients with DCM than that of the control group ([1.55±0.55]% ),P<0.01. And the frequency of CD4~+CD25~+Foxp3~+ T cells had negative correlation with heart function grade((NYHA) (r=0.693, P<0.01).Conclusions:The frequency of regulatory T cells decrease in patients with DCM and is associated with aggravated heart function. The reduction of regulatory T cells may lead to the down-regulation of autoimmune tolerance and bring about autoimmunity cardiomyopathy.