枸杞多糖对高脂血症大鼠血脂代谢及氧自由基的影响

目的:探讨枸杞多糖(LBP)对高脂血症大鼠血脂代谢及氧自由基的影响.方法:健康雄性Wistar大鼠60只,按体重随机分为空白对照组、高脂血症模型组、LBP高、中、低剂量组和阳性对照组；除空白对照组,其余各组采用高脂饲料喂养法建立大鼠高脂血症模型；模型建立成功后,空白对照组和模型组ig给予蒸馏水,其余各组分别灌胃给予LBP(80,40,20 mg·kg-1)及辛伐他汀2 mg· kg-1.4周后,测定血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白(HDL-C)、超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)水平.结果:与高脂血症模型组比较,LBP各剂量组大鼠血清TC,TG,LDL-C均显著下降(P＜0.05或P＜0.01),而HDL-C则显著升高(P＜0.05)；SOD,GSH-Px活性显著升高(P ＜0.05或P＜0.01),而MDA水平显著下降(P＜0.05或P＜0.01).结论:LBP能调节高脂血症大鼠血脂代谢水平,清除氧自由基,防止脂质过氧化,延缓动脉粥样硬化形成.

Effects of Lycium barbarum Polysaccharides on Lipid Metabolism and Oxygen Free Radicals in Hyperlipidemic Rats

Objective:To study the effects of Lycium barbarum polysaccharides (LBP) on lipid metabolism and oxygen free radicals in hyperlipidemic rats. Method: Sixty Wistar rats were randomly divided into six groups: normal control group, high-lipid model group, LBP high, medium and low-dose group and positive control group. The rat model of hyperlipidemic was established by high-fat diet feeding. After 4 weeks, the rats were given with LBP (80, 40, 20 mg·kg^-1) and simvastatin 2 mg·kg^-1. At the end of 4 weeks, the abdominal aortic blood was collected and determined total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px). Result: Compared with hyperlipidemia model group, serum TC, TG, LDL-C level in each treatment group decreased significantly (P<0.05 or P<0.01), HDL-C significantly increased (P<0.05); SOD and GSH-Px activity significantly increased (P<0.05 or P<0.01), MDA levels decreased significantly (P<0.05 or P<0.01). Conclusion: LBP could adjust the level of lipid metabolism of rat model of hyperlipidemia, inhibit the damage from oxygen free radicals, prevent lipid peroxidation, and delay atherosclerotic formed.