氢醌诱导下肝细胞XPV基因mRNA表达规律的研究

目的研究氢醌(HQ)对L-02肝细胞XPV基因mRNA表达的影响及其规律,探讨XPV基因在HQ所致肝细胞毒性过程中分子作用机制。方法体外培养的L-02肝细胞经40μmol/L的HQ诱导后,采用实时荧光定量PCR法检测不同时间点(6、12、24和48h)XPV基因mRNA的表达;并检测不同剂量(0、5、10、20、40、80和160μmol/L)HQ诱导24h后,各浓度组XPV基因mRNA的表达。结果在24h的时间范围内,XPV基因在mRNA水平上的表达无论是染毒组(40μmol/L)还是正常对照组,均具有随时间的延长而增加的趋势;到24h时,相对表达量达到最大值,当培养时间达到48h时,该基因的表达则有所下降;此外,在各时间处理组(6h、12h、24h和48h)中,染毒组L-02肝细胞内XPV基因在mRNA水平上的表达均高于正常对照组。各剂量HQ作用24h后,L-02肝细胞内XPV基因mRNA的表达在0～80μmol/L范围内随着剂量的增加而增加,以正常对照组XPV基因的相对表达量为1,各染毒剂量组内XPV基因mRNA的相对表达量分别增至1.20、2.02、2.37、2.67、4.40和2.32倍;80μmol/L时其相对表达量达到峰值,160μmol/L的HQ作用后,其表达却有所下降,但仍高于正常对照组。结论HQ可以诱导XPV基因mRNA的表达变化,并且呈现一定的时间和剂量依赖性,提示XPV基因可能参与了肝细胞对HQ的应答过程。

Study on expression regularity of XPV mRNA in L-02 hepatic cells induced by hydroquinone

Objective To study the effect of hydroquinone(HQ) on expression of XPV mRNA in L-02 hepatic cells and its regularity,in order to explore the possible molecular mechanism of XPV in the process of the toxic effects of HQ to L-02 hepatic cells.Methods Expression of XPV mRNA in L-02 hepatic cells treated at the dose of 40μmol/L HQ for 6,12,24 and 48h in vitro and its expression in L-02 hepatic cells treated at the concentrations of 0,5,10,20,40,80 and 160μmol/L HQ for 24h was measured by real-time fluorescence quantitative PCR.Results In the range of 0-24h,the expressions of XPV mRNA in L-02 hepatic cells in treated group(40μmol/L) and control group increased with increase of time,and expressions of XPV reached the maximum levels at the times of 24h,and reduced the minimum levels at the times of 48h.At the time ranges of 6,12,24 and 48h,the expression levels of XPV mRNA in L-02 hepatic cells treated with HQ(40μmol/L) were more higher than those of the control(0μmol/L).The expression level of XPV mRNA in L-02 hepatic cells treated by HQ at the concentration of 0-80μmol/L for 24h increased in a dose-dependent manner.Taken the expression level of the control group as 1,the relative expression levels in various treatment groups were added to 1.20,2.02,2.37,2.67,4.40 and 2.32 fold respectively.The expressions of XPV mRNA in the group treated with HQ(160μmol/L) was reduced,but was higher than that of the control.Conclusion HQ could induce the expression of XPV at mRNA levels in a dose-and time-dependent manner,which indicated that XPV could involved in the response of L-02 hepatic cells of HQ.