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| 复合酶法提取毛脉酸模中有效成分的工艺研究 | |
| 引用本文： | 刘颖新,王振月,刘利利,王谦博.复合酶法提取毛脉酸模中有效成分的工艺研究[J].时珍国医国药,2011,22(11):2601-2603. |
| 作者姓名： | 刘颖新 王振月 刘利利 王谦博 |
| 作者单位： | 1. 黑龙江中医药大学药学院,黑龙江哈尔滨150040;湖南中医药高等专科学校,湖南株洲412012 2. 黑龙江中医药大学药学院,黑龙江哈尔滨,150040 3. 湖南中医药高等专科学校,湖南株洲,412012 4. 黑龙江省药品检验所,黑龙江哈尔滨,150010 |
| 摘 要： | 目的采用复合酶法提取毛脉酸模中有效成分,研究其提取最佳工艺。方法以毛脉酸模根中白藜芦醇苷、白藜芦醇、大黄酚苷、酸模素、大黄素、大黄酚、大黄素甲醚及出膏率为考察指标,采用多指标综合评分和正交设计实验优化复合酶法提取毛脉酸模有效成分的工艺。结果复合酶法提取毛脉酸模有效成分的最佳酶解条件:酶量为3%,酶解时间120min,酶解温度40℃,pH=6。结论验证试验结果表明该提取工艺稳定可行,适用于毛脉酸模有效成分的提取。 |
| 关 键 词： | 复合酶法 毛脉酸模 有效成分 |
The Technology of Extracting Effective Components from Rumex gmelini by Compound Enzymolysis Method |
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| LIU Ying-xin,WANG Zhen-yue,LIU Li-li,WANG Qian-bo.The Technology of Extracting Effective Components from Rumex gmelini by Compound Enzymolysis Method[J].Lishizhen Medicine and Materia Medica Research,2011,22(11):2601-2603. | |
| Authors: | LIU Ying-xin WANG Zhen-yue LIU Li-li WANG Qian-bo |
| Institution: | LIU Ying-xin 1,2,WANG Zhen-yue 1*,LIU Li-li2,WANG Qian-bo3 (1.Heilongjiang University of Traditional Chinese Medicine,Haerbin 150040,China,2.Hunan Traditional Chinese Medical College,Zhuzhou 412012,3.Heilongjiang Drug Inspection Institute,China) |
| Abstract: | Objective To extract effective components from Rumex gmelini Turcz by compound enzymolysis method and research the optimal technology. Methods Using resveratrol glucoside,resveratrol,chrysoretin,musizin,emodin,chrysophanol,physcion of Rumex gmelini Turcz and rate of creaming as indexes.The orthogonal design experiment and multiple guidelines grading method were adopted to optimize extracting process. Results The optimum conditions were as follows:compound enzyme amount 3%,enzymatic extraction time 120 min,e... |
| Keywords: | Compound enzymolysis method Rumex gmelini Turcz Effective components |
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