盐酸雷尼替丁胶囊中盐酸雷尼替丁的微流控芯片测定

目的建立微流控芯片测定盐酸雷尼替丁胶囊中盐酸雷尼替丁含量的方法。方法在高电场强度条件下利用微流控芯片上特殊的微通道实现组分快速、高效的分离,以非接触电导检测器进行检测;研究电泳介质、分离电压、进样时间等因素对分离检测的影响。结果优化选择2 mmol.L-1 HAc+2 mmol.L-1 NaAc(pH=4.5)+0.2 mmol.L-1 SDS为电泳介质,在分离电压为2.00 kV,进样时间为10 s时,1 min内可实现盐酸雷尼替丁快速分离检测;在优化条件下,盐酸雷尼替丁的线性范围为10～110μg.ml-1(r2=0.999 6),检出限为1.3μg.ml-1(S/N=3),RSD为1.6%,加标回收率为99.1%～101.2%。结论该方法简便、快速、灵敏,重复性较好,可用于盐酸雷尼替丁制剂的质量控制。

Content Determination of Ranitidine Hydrochloride in Ranitidine Hydrochloride Capsules by Microfluidic Chip

ObjectiveTo establish a new method for the determination of ranitidine hydrochloride in ranitidine hydrochloride capsules by microfluidic chip. MethodsRanitidine hydrochloride was rapidly separated by microfluidic chip and detected with contactless conductivity detection under high electric field.The electrophoretic parameters,such as the variety and concentration of buffer solution and additive,separation voltage and injection time etc,were researched. ResultsIn optimal conditions,using 1 mmol·L-1 HAc + 2 mmol·L-1 NaAc(pH = 4.5) as buffer solution,with 0.2 mmol·L-1 SDS at the separation voltage of 2.00 kV and 10s injection,ranitidine hydrochloride was separated and detected within 1 min.The linear dependence of the concentration of ranitidine hydrochloride ranged from 10 μg·ml-1 to 110 μg·ml-1(r2=0.999 6),the detection limit was 1.3 μg·ml-1(S/N=3),the RSD was 1.6% and the recoveries ranged from 97.6% to 98.6%. ConclusionThe method is simple,rapid,sensitive and well reproducible,and it can be used in the quality control of the product.