白毛藤生物碱对小鼠免疫功能及部分细胞因子mRNA表达的影响

 目的探讨白毛藤生物碱对小鼠免疫功能及细胞因子IL-2,IL-12,IFN-γ和TNF-αmRNA表达的影响。方法将BALB/c小鼠随机分成4组:白毛藤生物碱高(H组)、中(M组)、低(L组)3个剂量组和生理盐水对照组(C组),连续腹腔注射7d后,检测其单核巨噬细胞吞噬功能、二硝基氟苯(DNFB)诱导的小鼠迟发型变态反应(DTH)、胸腺指数和脾脏指数,并用RT-PCR的方法检测脾脏IL-2,IL-12,IFN-γ和TNF-αmRNA表达水平。结果L组和M组的廓清指数(K)、吞噬指数(α)、DNFB诱导的小鼠迟DTH、胸腺指数和脾脏指数均显著高于C组,H组K和α显著低于C组;白毛藤生物碱3个剂量组均能提高IL-2,IL-12,IFN-γ和TNF-αmRNA表达水平,与C组相比,M组最显著。结论白毛藤生物碱能有效提高机体的免疫功能及IL-2,IL-12,IFN-γ和TNF-αmRNA表达。

Effects of Solanum lyratum Thunb alkaloids on immunity and mRNA expression of some cytokine in mice

OBJECTIVE To investigate the effects of Solanum lyratum Thunb alkaloids on immunity and mRNA expressions of some cy-tokines in mice.METHODS Balb/c mice were divided into 4 groups: the mice in control group were treated with saline(C) .The mice in other 3 groups, were treated with high( H), moderate(M) and low(L) dosage Solarium lyratum Thunb alkaloids, respectively. The 4 groups were administered for 7 d. The phagocytosis of mononuclear macrophage, DTH induced by DNFB, thymus index and spleen index were determined. The mRNA expression of IL-2,IL-12,IFN-γ and TNF-α were examined by RT-PCR at the 8th day.RESULTS The levels of carbon particle clearance index, phagocytic index, DTH induced by DNFB, thymus index and spleen index in L group and M group were significantly higher than that in normal saline group(C) .The levels of carbon particle clearance index and phagocytic index in H group were significantly lower than that in normal saline(C). The levels of IL-2,IL-12,IFN-γ and TNF-α mRNA expression in 3 groups injected with Solanum lyratum Thunb alkaloids were increased, the level in M group was the highest. CONCLUSION Solanum lyratum Thunb alkaloids can enhance immunity and mRNA expressions of IL-2, IL-12,IFN-γ and TNF-α in mice.