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等电聚焦电泳分析红细胞Gd(-)壮族富宁型〔Gd(-)Zhuang-Funing〕
引用本文:刘泽源,田兴亚,周秀贞. 等电聚焦电泳分析红细胞Gd(-)壮族富宁型〔Gd(-)Zhuang-Funing〕[J]. 昆明医学院学报, 1991, 0(2)
作者姓名:刘泽源  田兴亚  周秀贞
作者单位:昆明医学院生物化学教研室,昆明医学院生物化学教研室,昆明医学院生物化学教研室
摘    要:本文采用聚丙烯酰胺凝胶等电聚焦电泳(IEF),借助园盘电泳仪的简易装置,对正常 B 型G6PD 及变异型 G6PD 进行分析,获得了满意效果。

关 键 词:等电聚焦电泳  等电点  G6PD 变异型

Characterization of Erythrocyte Gd(-)Zhuang-Funing by Isoelectric Focusing
Liu Zeyuan. Characterization of Erythrocyte Gd(-)Zhuang-Funing by Isoelectric Focusing[J]. Journal of Kunming Medical College, 1991, 0(2)
Authors:Liu Zeyuan
Affiliation:Department of biochemistry
Abstract:The existence of microheterogeneity of glucose-6-phosphate dehydrogenase(G6PD)in the human erythrocyt has already been reported(Der Kaloustian 1974).The results has been confirmed recently by isoelectric focusing in polyacrylamide gel.In this paper,we used the method which has been modified in some aspects to identify various G6PD variants,and came to interesting conclusions.We provide here our experimental data of two different G6PD types,GdB and Gd(-)Zhuang-Funing,Focusing of the enzyme gives additional in- formation concerning an accurate distinction among the genetic variants.
Keywords:Isoelectrofocusing electrophoresis  Isoelectric point  G6PD variant
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