rhBMP-7对大鼠成骨细胞增殖及分化能力的影响

目的研究重组人骨形态发生蛋白-7（recombinant human bone morphogenetic proteins，rhBMP-7）对大鼠成骨细胞增殖和分化能力的影响。方法从SD大鼠取材进行原代培养，经碱性磷酸酶（alkaline phosphatase，ALP）和矿化结节检测鉴定为成骨细胞后传代至第3代，接种至96孔培养板。分为A、B、C、D、E、F共6组，每组3个复孔，rhBMP-7浓度分别为0．000、0．500、1．000、5．000、10．000、50．000mg/L。应用四甲基偶氮唑盐（methyl thiazolyl tetrazolium，MTT）法及PNPP偶氮法，分析不同浓度rhBMP-7作用72h后对大鼠成骨细胞增殖和ALP活性的影响，应用单因素方差分析和LSD法统计数据。结果作用72h后，6种浓度rhBMP-7对大鼠成骨细胞ALP活性促进作用的差异有统计学意义（F=11．840，P=0．000），B组成骨细胞ALP活性高于A组，但差异无统计学意义（P=0．078），C、D、E、F组大鼠成骨细胞ALP活性均高于A组（P值均为0．000），rhBMP-7浓度为50．000mg／L时对ALP活性的影响最明显（P=0．000）。作用72h后6种浓度rhBMP-7对大鼠成骨细胞增殖影响的差异有统计学意义（F=2．726，P=0．026）；rhBMP-7能促进大鼠成骨细胞的增殖和分化，同A组（rhBMP-7浓度为0．000mg／L）相比，当rhBMP-7浓度为50．000mg／L时并作用至第3天时，大鼠成骨细胞增殖最显著（P=0．000）。结论一定剂量的rhBMP-7明显促进了大鼠成骨细胞的增殖和分化。

Effect of Recombinant Human Bone Morphogenetic Protein-7 on the Proliferation and Differentiation of Rat Osteoblast

Objective To study the effect of recombinant human bone morphogenetic protein-7 （rhBMP-7） on the proliferation and differentiation of osteoblasts. Methods Cell culturing, the light microscopic, MTT assay and PNPP assay were used to observe the effects of rhBMP-7 on proliferation and differentiation in the cell morphology and the cellular cytomety of proliferation and differentiation. Results rhBMP-7 could stimulate osteoblasts proliferation and differentiation. The maximal effect on proliferation was obtained at a concentration of 50. 000 mg/L and in the third day. ALP activity in osteoblasts were increased most significandy also at the same concentration of rhBMP-7. Conclusion rhBMP-7 may stimulate bone formation in a direct fashion by induction of proliferation and differentiation of osteoblasts.