血小板源生长因子受体结合域基因与乙型肝炎核心抗原基因融合克隆的构建

血小板源生长因子在动脉粥样硬化发生中起着重要的作用为了阻断血小板源生长因子致动脉粥样硬化的作用，我们利用基因工程技术试图制备血小板源生长因子受体结合域与乙型肝炎核心抗原的融合蛋白，并以此蛋白作为疫苗，探讨了基防治动粥样硬化发生发展是可能性，本文报道血小板源生长因子受体结合域基因的化学合成以及与乙型肝炎核心抗原基因的融合克隆的构建，经双酶切，序列分析证实，这两种基因融合成功。

Cloning of the Gene of a Fusion Protein-Platelet Derived Growth Factor Receptor Binding Domain with Hepatitis B Core Antigen

Aim Platelet derived growth factor (PDGF) plays important roles in the development of atherosclerosis.In order to block the effects of PDGF, PDGF receptor binding domain was fused with the hepatitis B core antigen (HBcAg) to form a fusion protein. This fusion protein can be used as a vaccine for the prevention of atherosclerosis in animal models. In this paper we report only the cloning of the fusion protein gene. Methods The gene of PDGF receptor binding domain, a 13 iner peptide, ANFLVWEIVRKKP, with 2 linkers (for Ndel and EcoRI) was synthesized chemically and cloned into a vector PBS. The HBcAg gene was obtained with polymerase chain reaction (PCR)technique and fused with the 13 iner peptide gene-pBS at the EcoRI and BamHI sites. Results A DNA fragment of about 600 hp in size was obtained. DNA sequencing analysis showed that the sequence of the 13iner peptide gene and the reading frame of the fused gene were both correct. Conclusion The gene of the fusion protein of the 13 iner peptide with HBcAg was successfully cloned in the vector PBS.