下咽鳞状细胞癌中Slit2的表达及其与微血管密度关系的临床研究

目的研究下咽鳞状细胞癌组织中神经迁移蛋白Slit2的表达及其与微血管密度的关系,探讨两者对下咽鳞状细胞癌的生物学行为的影响。方法免疫组织化学法检测Slit2蛋白的表达及微血管密度（MVD）,并应用RT PCR法检测新鲜组织中Slit2 mRNA的表达。研究其表达与肿瘤临床特征的关系。结果Slit2蛋白在下咽鳞状细胞癌和癌旁组织中的表达率分别为65.2%和40.5%,两者表达差异有统计学意义（χ2=6.329,P<0.05）;Slit2 mRNA在下咽鳞状细胞癌和癌旁组织中的表达,差异亦具有统计学意义（t=8.230,P<0.05）。Slit2蛋白的表达与肿瘤大小、TNM分期、淋巴结转移显著相关;MVD计数与肿瘤大小、浸润深度、TNM分期和淋巴节转移显著相关,两者与肿瘤的发病年龄、部位、临床病理分化程度均无关。肿瘤组织中Slit2阳性表达者MVD计数高于Slit2阴性表达者,其差异具有统计学意义（t=2.273,P<0.05）;并且两者表达呈正相关（rs=0.430,P<0.05）。结论下咽鳞状细胞癌组织中Slit2表达和MVD计数,与肿瘤的侵袭和淋巴结转移密切相关,其机制可能为Slit Robo信号系统介导了肿瘤新生血管的形成,从而影响了肿瘤的生物学行为。

Expression of Slit2 and its correlation with micro-vesseldensity in hypopharyngeal squamous cell carcinoma

To investigate expression of Slit2 and its correlation with micro vessel density in human hypopharyngeal squamous cell carcinomas, and to analyze their influences on the biological behavior of human hypopharyngeal squamous cell carcinoma. MethodsExpressions of Slit2 and CD34 labeled micro vessel density (MVD) were measured by immunohistochemical staining (Envision), and expression of Slit2 mRNA in 24 cases of fresh tissues was determined by reverse transcript polymerase chain reaction. ResultsThe positive rates of Slit2 in cancerous tissues and para cancerous tissues were respectively 65.2% and 40.5%（χ2=6.329,P<0.05）. Slit2 mRNA expressions between them also had statistical significance (t=8.230, P<0.05). Expression rate of Slit2 and enumeration of MVD were significantly associated with tumor size,TNM stage, and lymph node metastasis. Enumeration of MVD was increased in Slit2 positive tissues（t=2.273, P<0.05）. Expression of Slit2 had a positive correlation with MVD（rs=0.430,P<0.05）. ConclusionExpression of Slit2 and enumeration of MVD are significantly higher in hypopharyngeal squamous cell carcinoma than in normal mucosa. They have significant relations to tumor invasion and lymph node metastasis,and the mechanism might be that Slit2 mediated new vessel formation in tumors.