核转录因子与细胞周期蛋白D1在宫颈癌中的表达及相关研究

目的 探讨核因子NF-κ B p65与细胞周期蛋白D1(cyclinD1)在宫颈癌中的表达及期与肿瘤增殖之间的关系。方法 应用免疫组织化学检测NF-κ B p65，cyclinD1在32例宫颈组织及30例正常宫颈组织中的表达情况。结果 宫颈肿瘤中NF-κ B p65蛋白阳性表达率为68．8％(22／32)，cyclinD1 78．5％，30例正常宫颈组织中NF-κ B无1例阳性表达，cyclinD1表达率为18．5％。结论NF-κ B p65在宫颈癌中异常活化，并与cyclinD1过度表达密切相关，NF-κ B p65可能通过上网cyclinD1表达参与宫颈癌的发生与发展。

Significance of NF-κB p65 and cyclin D1 protein expression in Human Cervical Squamous cell carcinoma proliferation

[Objective] To evaluate the significance of expression of nuclear factor kappa B (NF-κB) p65 protein and cyclinD1 and their relationship with cervical carcinoma proliferation. [Methods] The expression of NF-κB p65protein and cyclin D1 protein in 32 cases of Cervical Squamous cell tumor tissue and 30 cases of normal cervical tissue were studied immunohistochemically. [Results ] None of the normal tissues exhibited NF-κBp65, In contrast,22 of 32 Cervical Squamous cell carcinoma specimens were positive for p65 protein with a 68.7% positive rate. Cervical cancer tissue were 78.5 % expression for cyclin D1 protein, normal cervical tissue were 18.5% expression for cyclin D1 protein. The NF-κB p65 protein, cyclin D1 protein expression were correlated to the differentiation (P ＜0.05 ), The expression of NF-κB a65 was closely related with cyclin D1. [Conclusion] NF-κB p65 is a new oncogenic protein related to the cervical tumors. NF-κB up-regulates cyclin D1, then leads to tumor cell malignant proliferation and tumor occurrence.