活体细胞凋亡检测筛选肝癌敏感化疗药物的实验研究

目的:通过动物实验探讨99 Tcm-HYNIC-Annexin V活体细胞凋亡检测筛选肝癌敏感化疗药物的可行性。方法:20只VX-2荷瘤免随机分为4组,通过治疗剂量的紫杉醇(PAC,n=6),5-氟尿嘧啶(5-FU,n=5),表柔比星(EPI,n=5)分组干预治疗,同时建立对照组(C,n=4)。分别予化疗前2h、化疗后46h静脉注射99 Tcm-HYN-IC-Annexin V,2h后再以单光子发射计算体层摄影(SPECT)成像,选取肿瘤部位放射性计数(T)及腿部非肿瘤区部位放射性计数(NT),计算其比值(T/NT),进行半定量分析,确定敏感化疗药物。取肿瘤组织,分别予免疫组化(Tunel)和流式细胞仪(FCM)分析组织细胞凋亡率,放射性核素聚集显像与肿瘤细胞凋亡进行相关性分析。实验数据以SPSS 17.0软件分析。结果:99 Tcm-HY-NIC-Annexin V放射显像显示,化疗前各组肿瘤部位均无明显显像(F=0.025,P=0.980),放射性核素分布主要聚集在肾脏及膀胱区。药物干预48h后,对照组仍无显像,而药物干预组显像清楚,放射性计数显著高于对照组(F=16.52,P<0.001)及化疗前(P<0.01)。药物干预组中,PAC组肿瘤组织放射性显像显著高于5-FU组和EPI组(P值分别为0.042和0.003),而5-FU组与EPI组间放射性显像差异无统计学意义(P=0.224)9。9 Tcm-HYNIC-Annexin V活体细胞凋亡检测结果与免疫组化及流式细胞检测结果一致(R2=0.537 7,P=0.002;R2=0.695 4,P<0.001)。结论9:9 Tcm-HYNIC-Annexin V核素显像检测能够反映活体组织细胞凋亡情况,该方法能够在活体状态下筛选敏感化疗药物。

Screening sensitive chemotherapeutic drug for liver cancer by apoptosis detecting in vivo

OBJECTIVE:To investigate the feasibility of screening sensitive chemotherapeutic drugs for liver cancer by 99Tcm-HYNIC-Annexin V detecting apoptosis in vivo.METHODS: New Zealand rabbits implanted VX-2 in liver were divided into control(C,n=4),paclitaxel(PAC,n=6),5-Fluorouracil(5-FU,n=5),and Epirubicin(EPI,n=5) group.Imagings of the liver tumor were detected by SPECT through intravenous injection 99Tcm-HYNIC-Annexin V before and after treatment for 48 h.Tumor radioactive count proportion to non-tumor sites(T...