腺病毒介导的心肌营养素-1基因转染对神经干细胞作用的实验研究

目的:观察腺病毒介导的心肌营养素-1(Adv-CT-1)基因转染对大鼠神经干细胞(NSCs)的作用。方法:分离、培养胚胎大鼠NSCs,以Adv-CT-1基因转染NSCs,应用细胞集落与细胞突起计数、流式细胞仪凋亡检测等技术,检测NSCs的生长与凋亡情况。结果:Adv-CT-1基因转染培养大鼠NSCs可增加NSCs集落数量和诱导分化细胞突起的数量(P<0.05)。在H2O2诱导的NSCs凋亡模型中,转染Adv-CT-1基因的NSCs凋亡细胞数量较对照组减少(P<0.05),存活细胞数增加(P<0.01)。结论:CT-1基因转染可促进NSCs分裂增殖以及细胞突起的生长,减少超氧化损伤诱导的NSCs凋亡发生,促进损伤NSCs的存活。

An experimental study of effects of adenovirus-mediated cardiotrophin-1 gene transfer into neural stem cells

Objective:To investigate the effect of adenovirus-mediated cardiotrophin-1(Adv-CT-1) gene transfer on rat neural stem cells(NSCs) in vivo.Method:NSCs were isolated,cultured and transferred with Adv-CT-1.The effects of Adv-CT-1 on NSCs were investigated by neuron-sphere growth and neurites assays.Adv-CT-1 was transferred into the injured NSCs,effects of Adv-CT-1 on NSCs survivance and apoptosis were checked in a H2O2-induced NSCs apoptosis injury model.Result:NSCs were cultured with conditional medium and persisted existing even after several passages.After transferring NSCs with Adv-CT-1,the colong and induced NSCs increased(P<0.05).CT-1 relieved NSCs apoptosis(P<0.05),improved cell survivance in H2O2-induced NSCs apoptosis model(P<0.01) which determined by flowcytometry.Conclusion:After transfection,Adv-CT-1 improved growth of NSCs and proliferation of neuritis,improved cell survivance in H2O2-induced NSCs apoptosis.