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| 还脑益聪方对早期认知功能障碍大鼠海马病理形态、炎症因子和氧化应激指标的影响 | |
| 引用本文： | 姚明江,李浩,赵文明,刘剑刚,徐立,官杰,蔡琳琳. 还脑益聪方对早期认知功能障碍大鼠海马病理形态、炎症因子和氧化应激指标的影响[J]. 世界中西医结合杂志, 2010, 5(4): 300-304 |
| 作者姓名： | 姚明江 李浩 赵文明 刘剑刚 徐立 官杰 蔡琳琳 |
| 作者单位： | 中国中医科学院西苑医院,北京,100091 |
| 基金项目： | 国家自然基金项目，国家自然基金项目，国家中医药管理局科技专项资助项目 |
| 摘 要： | 目的观察还脑益聪方对复合因素所致早期认知功能障碍大鼠行为学及海马病理形态、炎症因子和氧化应激指标的影响。方法采用皮下注射D-半乳糖及饲喂半高脂饲料的方法造成早期认知功能障碍大鼠模型,并以还脑益聪方灌胃治疗。采用Morris水迷宫试验观察大鼠行为学变化,HE染色及透射电镜观察海马病理形态学改变;应用放射免疫法检测大鼠海马匀浆白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量,酶联免疫法(ELISA法)测定氧化低密度脂蛋白(ox-LDL)含量,免疫比色法测定丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)和谷胱甘肽S转移酶(GSH-ST)含量。结果还脑益聪方能增加模型大鼠水迷宫试验穿台次数(P0.01),减轻模型大鼠海马神经细胞损伤、线粒体病变及胶质细胞增生,降低海马匀浆TNF-α及ox-LDL含量(P0.05,P0.01)。结论还脑益聪方能提高早期认知功能障碍模型大鼠的空间学习记忆能力,这可能与其抑制脑内炎症反应与氧化应激,从而减轻海马组织病理变化有关。 |
| 关 键 词： | 还脑益聪方 认知功能障碍 行为学 病理 炎症 氧化应激 |
Effect of Huannao Yicong Formula on Pathomorphology,Inflammatory Factor and Oxidative Stress Index of Hippocampus in Cognitive Impairment Rats at Early Stage |
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| YAO Ming-jiang,LI Hao,ZHAO Wen-ming,LIU Jian-gang,XU Li,GUAN Jie,CAI Lin-lin. Effect of Huannao Yicong Formula on Pathomorphology,Inflammatory Factor and Oxidative Stress Index of Hippocampus in Cognitive Impairment Rats at Early Stage[J]. World Journal Of Integrated Traditional and Wesrern Medicine, 2010, 5(4): 300-304 | |
| Authors: | YAO Ming-jiang LI Hao ZHAO Wen-ming LIU Jian-gang XU Li GUAN Jie CAI Lin-lin |
| Affiliation: | YAO Ming-jiang,LI Hao,ZHAO Wen-ming,LIU Jian-gang,XU Li,GUAN Jie,CAI Lin-lin (Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091) |
| Abstract: | Objective To observe the effect of huannao yicong formula on pathomorphology,inflammatory factor and oxidation stress index of hippocampus in cognitive impairment rats at early stage induced by compound factors.Methods The cognitive impairment rat models were induced by hypodermic D-galactose injection and half fat feeder,and were treated with huannao yicong formula via intragastric administration.Morris water maze was used to observe the praxiological changes of rats.HE staining and transmission electron m... |
| Keywords: | Huannao Yicong Formula Cognitive Impairment Praxiology Pathomorphology Inflammation Oxidative Stress |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |