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| 高效液相色谱-蒸发光检测器测定食品中的三氯蔗糖 | |
| 引用本文： | 鲁琳,杭义萍,高燕红. 高效液相色谱-蒸发光检测器测定食品中的三氯蔗糖[J]. 中国热带医学, 2008, 8(9): 1628-1629 |
| 作者姓名： | 鲁琳 杭义萍 高燕红 |
| 作者单位： | 1. 广东省疾病预防控制中心,广东,广州,510300;华南理工大学,广东,广州,510640 2. 华南理工大学,广东,广州,510640 3. 广东省疾病预防控制中心,广东,广州,510300 |
| 基金项目： | 广东省医学科学技术研究基金 |
| 摘 要： | 目的建立食品中三氯蔗糖含量测定方法，并对前处理进行优化。方法采用高效液相色谱仪（HPLC），蒸发光检测器（ELSD），SUPELCO Discovery C18 4．6×150mm色谱柱，柱温：35℃，流动相由乙腈和超纯水组成，流速为1．0ml／min，蒸发管温度：4JD℃，气体压力3．5bar，放大倍数为6。结果采用HPLC—ELSD方法，其工作曲线的线性范围：0．1005～0．8040mg／ml，相关系数：r=0．99993，回收率：94．3％～97．1％，精密度（n=6）：1．9％～2．3％。结论方法准确、可靠，可用于三氯蔗糖的检测。 |
| 关 键 词： | 三氯蔗糖 高效液相色谱 蒸发光检测器 |
Determination of sucralose in food by HPLC-ELSD |
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| LU Lin,HANG Yi-ping,GAO Yan-hong. Determination of sucralose in food by HPLC-ELSD[J]. China Tropical Medicine, 2008, 8(9): 1628-1629 | |
| Authors: | LU Lin HANG Yi-ping GAO Yan-hong |
| Affiliation: | LU Lin, HANG Yi - ping, GAO Yan- hong. ( Postgraduate, Chemical Science College of Huanan Physical and Industrial University, Guangzhou 510640, Guangdong, P. R. China) |
| Abstract: | Objective To establish an optimized method for determination of sucralose in foods. Methods High performance liquid chromatography（ HPLC ） was used to determine sueralose in food respectively link with evaporative light seattering detector （ELSD） ,The column was SUPELCO Discovery C18 4.6 × 150mm, column temperature was 35℃, the mobile phase was made of aeetonitrile and water with flow rate 1.0ml/min,The temperature of evaporative pipe is 40℃ ,the gas pressure is 3.5bar, the zoom in mtdtiple is 6. Results The linear range of the calibration curve of HPLC - ELSD were from 0. 1005 - 0. 8040mg/mL（ r = 0.99993 ）. The recoveries were 94.3 - 97.1% and the relative standard deviations （ n = 6） were 1.9% - 2.3%. Conclusion The method was accurate,reliable, which can be used to determine sueralose in foods. |
| Keywords: | Sucralose High performance liquid chromatography Evaporative light scattering detectors |
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