低氧大鼠脑组织磷酸二酯酶活性的变化及意义

目的研究低氧时大鼠脑组织磷酸二酯酶(PDE)活性的变化及意义。方法18只SD大鼠随机分为正常对照组、窒息组和低氧-窒息组。低氧-窒息组6只予8%低氧气体吸入3h后窒息处死;窒息组6只予氮气窒息处死;正常对照组则用断头法处死。处死后迅速取脑制成脑组织酶样品,采用高效液相色谱法测定PDE活性。结果低氧-窒息组PDE活性为(230±28)nmol·g-1·min-1,明显低于正常对照组(365±67)nmol·g-1·min-1,而窒息组PDE活性(439±138)nmol·g-1·min-1则高于对照组,但差异无统计学意义。结论低氧时大鼠脑组织PDE活性可能存在一个时相的变化过程,PDE可能参与了缺氧性脑损伤的病理过程。

Changes and clinical significance of phosphodiesterase activity in rat brain during hypoxia

Objective To study the changes and clinical significance of phosphodiesterase (PDE) activity in rat brain during hypoxia. Methods Eighteen Sprague-Dawley rats were divided into hypoxia-asphyxia group,asphyxia group and control group at random.Hypoxia-asphyxia group(n=6) breathed in 8% oxygen for 3 hours and then were suffocated with nitrogen;asphyxia group(n=6) were suffocated with nitrogen;but controls(n=6) were killed by decapitation.All rats′brains were isolated for determining the PDE activities by HPLC. Results PDE activities in hypoxia-asphyxia group〔(230±28) nmol·g-1·min-1〕 were lower than those in controls 〔(365±67) nmol·g-1·min-1〕(P<0.01); but PDE activities in asphyxia group〔(439±138) nmol·g-1·min-1〕 were higher than controls (P>0.05). Conclusion There exists a phase-change for the PDE activities in rats′ brain during hypoxia,PDE may participate in the pathological course of brain injury during hypoxia.