| 反相高效液相色谱法测定5—氨基水杨酸相关物质、原料含量及缓释片含量 |
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| 引用本文： | 张蜀,易军,谭载友,周宏兵. 反相高效液相色谱法测定5—氨基水杨酸相关物质、原料含量及缓释片含量[J]. 中国医院药学杂志, 2001, 21(3): 139-141 |
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| 作者姓名： | 张蜀 易军 谭载友 周宏兵 |
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| 作者单位： | 广东药学院药物研究所，广东 广州 510224 |
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| 摘 要： | 目的：应用高效液相色谱法测定5-氨基水杨酸原料药及缓释片的含量。方法：反相高效液相色谱法，选择KromasilC18柱，以甲醇-磷酸盐缓冲液（35：65）为流动相，流速1ml.min^-1，检测波长254nm。结果：浓度与峰高的回归方程为Y=395.36 17.308X，相关系数r=0.9994，线性范围83.71-837.12ug.ml^-1。精密度RSD≤1.34%。辅料对制剂的含量测定无干扰，平均回收率99.25%，RSD=0.65%。结论：测定方法简便、准确、灵敏度高。
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| 关 键 词： | 反相高效液相色谱法 5－氨基水杨酸 缓释片 含量 测定 |
| 文章编号： | 1001-5213(2001)03-0139-03 |
| 修稿时间： | 2000-02-21 |
RP-HPLC determination of5-aminosalicylic acid |
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| ZHANG Shu,YI Jun,TAN Zai-You,et al. RP-HPLC determination of5-aminosalicylic acid[J]. Chinese Journal of Hospital Pharmacy, 2001, 21(3): 139-141 |
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| Authors: | ZHANG Shu YI Jun TAN Zai-You et al |
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| Abstract: | OBJECTIVE：RP-HPLC was employed in the determination of thecontent of material and extended release tablets of 5-aminosalicylic acid (5-ASA) and its extended release tablets.METHODS：RP-HPLC analysis was carried out by using Kromasil C18 columm,with methanol-phosphate buffer(35∶65) as mobile phase.Flow rate 1ml.min-1,detection wavelength 254nm.RESULTS：The regression equation of concentration and peak height was Y=359.36+17.308X,with r=0.9994,linear range from 83.71 μg.ml-1to 873.1 μg.ml-1,RSD of accuracy≤1.34%,and the average RSD of recovery was 0.65%.Additives had no disturbing on the determination of the preparation.CONCLUSIONS：The determination method is simple,accurate,and reliable. |
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| Keywords: | RP-HPLC 5-aminosalicylic acid(5-ASA) extended release tablets determination |
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