| 星形胶质细胞毒蕈碱样乙酰胆碱受体M2、M4亚型基因克隆及序列分析 |
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| 引用本文： | 张晓娟,汪海. 星形胶质细胞毒蕈碱样乙酰胆碱受体M2、M4亚型基因克隆及序列分析[J]. 中国临床药理学与治疗学, 2005, 10(10): 1108-1111 |
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| 作者姓名： | 张晓娟 汪海 |
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| 作者单位： | 军事医学科学院毒物药物研究所,北京,100850 |
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| 基金项目： | 国家自然科学基金(№30371641)军事医学科学院创新基金资助 |
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| 摘 要： | 目的：克隆胶质细胞M_2、M_4受体亚型基因序列，并比较胶质细胞M_2、M_4受体亚型基因序列和蛋白质序列与神经元细胞M_2、M_4受体基因序列和蛋白质序列间的差异。方法：根据神经元细胞M_2、M_4受体基因序列设计出针对M_2、M_4受体基因序列全长的特异性探针，采用RT-PCR方法扩增胶质细胞M_2、M_4受体亚型基因序列，并对其进行克隆测序。结果：通过RT-PCR方法扩增胶质细胞M_2、M_4受体亚型基因序列，与神经元细胞M_2、M_4受体比较，M_2受体差异碱基17个，发生氨基酸改变的有8个；M_4受体差异碱基3个，发生氨基酸改变的有2个。结论：胶质细胞M_2、M_4受体与神经细胞M_2、M_4受体亚型在基因序列和氨基酸序列上具有明显差异。
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| 关 键 词： | 非神经性胆碱系统 星形胶质细胞 基因克隆 聚合酶链式反应 序列分析 M受体 |
| 文章编号： | 1009-2501(2005)10-1108-04 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005-09-26 |
Sequence analysis of muscarinic receptors M2 and M4 expression by cultured rat astrocyte cells |
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| ZHANG Xiao-juan,WANG Hai. Sequence analysis of muscarinic receptors M2 and M4 expression by cultured rat astrocyte cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(10): 1108-1111 |
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| Authors: | ZHANG Xiao-juan WANG Hai |
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| Affiliation: | Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100580, China |
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| Abstract: | AIM: To clone the muscarinic receptor M_2, M_4 sequence of astrocyte cell, and compare the gene and protein sequence with those of neuron. METHODS: The specific primers were designed to clone the M_2 and M_4, sequence of astrocyte cell according to the sequences that of neuron by RT-PCR, and then the sequences were sequenced. RESHLTS AND CONCLUSIONS: The M_2 and M_4 sequences expression were compared by astrocyte cells and neurons, and the difference was marked. The differences of the gene and protein sequences are evident of M_2 and M_4 between astrocyte cell and neuron. |
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| Keywords: | non-neuronal cholinergic system astrocyte cell gene clone PCR sequence analysis muscarinic receptor |
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