Experimental study on the antitumor effect of chicken anemia virus vp3 gene against liver carcinomain vivo |
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Authors: | Zhifa Shen Yuzhe Wang Yiqiang Zong Shen Qu |
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Institution: | (1) Department of Biochemistry, School of Medical Sciences and Life Science, Jianghan University, 430051 Wuhan;(2) Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, 430030 Wuhan |
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Abstract: | Summary In order to testify the antitumor effect, especially its effect against liver carcinomain vivo, of VP3 protein, one kind of protein coded by chicken anemia virus, recombinants pcDNA-vp3 containing chicken anemia virus
vp3 gene, and control vector pcDNA3 were mixed with murine liver carcinoma cell lines H22 respectively. The mixture was injected
subcutaneously into Balb/C mice. Some days later, the mice were killed and the solid tumor weighed. The antitumor efficiency
was evaluated. The manners of VP3 proteinin vivo, inducing tumor cell death were identified by using TUNEL assay. All the results suggested that the injection of pcDNA-vp3
and H22 mixture resulted in a significant reduction of tumor growth in mice when compared with the results of control groups.
TUNEL assay revealed that VP3 induced apoptosisin vivo. All these indicated that CAV vp3 might be a potential new gene in reducing the growth rate of tumor cells in liver carcinoma
or in other kind of solid tumorsin vivo.
SHEN Zhifa, male, born in 1971 Lecturer |
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Keywords: | chicken anemia virus VP3 protein apoptosis gene therapy |
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