热应激预处理对梗阻性黄疸大鼠肝功能的影响

目的:研究热应激预处理对梗阻性黄疸大鼠肝功能的影响。方法:将30只Wistar大鼠随机分为3组,胆总管结扎组开腹分离并结扎大鼠的胆总管,假手术组只开腹分离但不结扎大鼠的胆总管,热应激预处理组在胆总管结扎手术前12h对大鼠行热应激预处理。术后1周处死大鼠穿刺心脏取血,检测各份血液标本的肝功能及胆红素含量。结果:胆总管结扎后1周,大鼠血胆红素明显升高,AST与ALT也随之明显升高,但热应激预处理组的AST与ALT升高幅度较小,与胆总管结扎组比较差异有统计学意义(P<0.05)。结论:热应激预处理可减轻梗阻性黄疸对大鼠肝脏的损害。

The Effect of Hyperthermia Pretreatment on The Liver Function of Obstructive Jaundiced Rats

Abstract Objective To study the effect of heat stress pretreatment on the liver function in rats with obstructive jaundice. Method 30 male Wistar rats were randomly divided into three groups : bile duct ligation group (B group):the common bile duct of the rat was divided and ligated; sham-operated group (S group): the common bile duct of the rat was divided but not ligated; Heat stress pretreatment group (P group): heat stress pretreatment was fisrt performed,12 hours later ligation of the common bile duct was followed.Each group had 10 rats. The rats were sacrificed one week after operation, blood samples from heart of the rats were measured for liver function. Results One week after ligation of the commom bile duct of the rats, ALT and AST of the rats in the B group and the P group significantly decreased when compared with those of the S group(P <0.01), while ALT and AST of the rats in the P group were significantly less than those of the B group(P <0.05). Conclusions Ligation of the commom bile duct will impair liver function of the rats, heat stress pretreatment can decrease the harmness to liver.