一种大鼠胰腺创伤模型的建立及伤情特点分析

目的 探讨胰腺创伤大鼠模型的建立方法及伤情特点.方法 应用BIM-Ⅲ型多功能生物撞击机产生压缩空气的方法,选择不同压力的冲击气流作用于开腹暴露的大鼠胰腺.观察压力作用后24h各压力组实验大鼠死亡率,一般状况变化,腹腔内变化及胰腺光镜、电镜下的病理变化,测定血清及腹水的淀粉酶、脂肪酶活性.分析各压力冲击气流作用下的各指标差异.结果 稳定建模的冲击压力上限为400kPa,在峰值压力为400kPa的冲击气流作用下,大鼠胰腺可发生明显损伤;在200kPa以下的冲击气流作用下,大鼠胰腺可发生轻度损伤.胰腺冲击气流损伤具有与急性胰腺炎的病情变化具有各种相同及不同的特征. 结论 该大鼠胰腺创伤模型建模过程简单,可重复性好,具有稳定的胰腺损伤的伤情特点,可用于进一步的病理机制和伤后救治的研究.

A pancreatic trauma model in rat and analysis of traumatic condition features

Objective To explore the establishment method of a pancreatic trauma model in rat,and to study the traumatic condition features of the model.Methods Rats were divided into three groups : the calibrate group,the experiment group and the control group.In the experiment group,blast air current with pressures of 800kPa,600kPa,400kPa,200kPa and 100kPa were acted to rat pancreas at the situation of abdomen operation respectively.The mortality,intra-abdominal structural changes,amylase and lipase activities of serum and ascites,and pathologic alterations under light and electron microscope of the pancreas were examined at 24h after trauma.Results The effectively maximal pressure of blast air current was 400kPa.Pancreatic edema,bleeding,cell necrosis and vacuolization were found obviously accompanied with marked increasing of amylase and lipase,but inflammation indexes were neglectable.Conclusion The pancreatic trauma model in rat has stable pancreas injury pathologic features with easily modeling process,fine repeatability.It is ideal for further pathologic and therapeutic study.