CAR在柯萨奇B3病毒感染心肌细胞中作用

目的探讨柯萨奇病毒和腺病毒受体(coxsackievirusandadenovirusreceptor,CAR)在嗜心性柯萨奇B3病毒(myocarditiccoxsaekievirusB3,CVB3m)感染心肌细胞中的作用。方法采用体外培养新生小鼠心肌细胞方法,建立CVB3m感染心肌细胞模型,将培养48h的心肌细胞分为对照组、CVB3m组和CAR抗体 CVB3m组,并作相应处理,继续培养48h后,分别观察各组心肌细胞病变效应(cytopathiceffect,CPE),并用四唑盐(MTT)比色法测定各组心肌细胞存活力。结果CAR抗体 CVB3m组心肌细胞CPE明显减轻,存活力显著增强,与CVB3m组比较差异显著(P<0.01),与对照组比较差异无显著性(P>0.05)。结论CAR抗体对CVB3m感染心肌细胞具有保护作用,提示CAR在CVB3m感染心肌细胞中可能发挥重要的介导作用。

The role of coxsackievirus and adenovirus receptor (CAR) on toxic cardiomyocytes infected by coxsackievirus B3

Objective To explore the role of coxsackievirus and adenovirus receptor (CAR) on toxie cardiomy-ocytes infected by myocarditic coxsackievirus B3 (CVB3m).Methods A toxic cellular model was established in vitro by adding CVB3m into the culture of neonatal mouse cardiomyocytes.48 hours after co- culturing, the cardiomyocytes were divided into control,CVB3m and CAR antibody CVB3m groups and processed correspondently.CVB3m- mediated myocyto-pathic effects on these three groups were observed after further co-culturing for 48 hours.At the same time,the cardiomyocytes' viability of these three groups was assessed with MTT assay.Results The degree of cytopathic effect (CPE) in CAR antibody CVB3m group decreased significantly compared with in CVB3m group(P<0.01) while significant increase of cell viability in CAR antibody CVB3m group in comparison with in CVB3m group(P<0.01 ).No significant differences was found between CAR antibody CVB3m group and control group (P>0.05).Conclusions CAR antibody has a protective effect on toxic cardiomyocytes infected by CVB3m, indicating that CAR may play an important role in mediating cardiotoxicity infected by CVB3m.