巢式PCR法检测健康人群人类细小病毒B19感染的研究

目的：探讨肇庆地区健康人群人类细小病毒Ｂ１９感染状况，方法：采用巢式聚合物酶链反应（Ｎｅｓｔｅｄ－ＰＣＲ）技术包括对４１１名新生儿，１８６名婴幼儿童，３７４名成人，６８９名孕妇的健康人１６６２名的脐血或指端，静脉血检测Ｂ１９ＤＮＡ。结果：１６６２名Ｂ１９阳性检出率为８．７２％，无性别差异，其中正常新生的Ｂ１９阳性检出率１０．７１％接近孕妇１０．２７％，均高于正常成人的５．０８％，（Ｐ〈０．０１），

Nested-PCR detection of human parvovirus B19 in healthy people

Objective:To study the prevalence of haman parvovirus B 19 infection in healthy resident population in Zhaoqing.Methods:Human parvovirus B 19 DNA from umbilical or finger-tip or venous blood was detected with nested-PCR in 1 662 cases of healthy subjects,icluding 411 newborns,186 infants,374 normal adults and 689 pregnant women.Results:The positive detective rates were 8.72% in all subjects,10.71% in newborns,10.27% in pregnant women and 5.08% in normal adults,respectively.The newborns and pregnant women had higher positive detective rate than adults(P<0.01),but no significant difference existed between infants and adults,and no sex difference was found.Conclusions:The positive infection rate of parvovirus B 19 in healthy population ranges from 5.08% to 10.71%,with higher rate in newborns and pregnant women. Owing to lower immunity and resistance to infeeton as physiological cause. The study suggests that multiple pregnancy shoud be prevented,and the policy of family planning and aristogenesis should be adopted.