邻苯二甲酸二丁酯对对羟基苯甲酸酯类雌激素活性效应的增强作用

目的评价对羟基苯甲酸丙酯(PP)、对羟基苯甲酸丁酯(BP)和邻苯二甲酸二丁酯(DPB)的单独和联合处理的雌激素活性效应。方法应用未成熟雌性Wistar大鼠连续3天皮下染毒的子宫增殖试验。通过重量及系数,判定PP、BP和DPB的单独处理和两两联合处理后的雌激素活性效应。结果实验中PP和BP雌激素活性最小可观察作用剂量(LOEL)分别为400mg/kg和200mg/kg,DPB在400~100mg/kg范围内没有发现雌激素活性。PP+BP等效应剂量配比联合处理中1LOEL和1/2LOEL具有子宫增殖作用,1/4LONEL未发现子宫增殖作用。PP和BP分别与子宫增殖试验中未见雌激素效应的DBP进行联合处理,在100mg/kgPP(1/4LOEL)+400mg/kgDBP,200mg/kgPP(1/2LOEL)+400mg/kgDBP和100mg/kgBP(1/2LOEL)+400mg/kgDBP联合处理下的子宫增殖试验出现雌激素活性。结论DBP具有增加PP和BP雌激素活性的效应,并且DBP对PP的雌激素活性的增强作用大于对BP的雌激素活性增强作用。

Enhancement of di-n-butyl phthalate on the estrogenic activities of esters of p-hydroxybenzoic acid

Objective To studied the estrogenicity of single propylparaben (PP), butylparaben (BP), di-n-butyl phthalate (DBP), and their joint treatment . Methods Estrogenicity was studied using the uterotrophic assay of immature female Wistar rats. Animals were subcutaneously (sc) treated for three consecutive days with single PP, BP, DBP and their joint treatment. Estrogenic activity effects were identified through the ratio of uterus/body weight. Results Estrogenic activities of PP and BP was detected, the lowest observed effect level (LOEL) of PP and BP were 400mg/kg and 200mg/kg, respectively, estrogenic activity of DBP was not detected at the doses of 400mg/kg and 100mg/kg. In the equal effect dosage joint treatment of PP and BP, uterus proliferation effects were observed at the doses of 1 and 1/2 LOEL, but the effect was not observed at the doses of 1/4 LOEL. In the joint treatment with DBP increased uterus proliferation effects of PP or BP were observed at matches of 100mg/kg PP(1/4LOEL) 400mg/kg DBP, 200mg/kg PP(1/2LOEL) 400mg/kg DBP and 100mg/kg BP(1/2LOEL) 400mg/kg DBP. Conclusion It was suggested that the DBP could have potential enhancing action to estrogenic activities of PP or BP, and the estrogenic activity of PP enhancement by DBP could be stronger than that of BP.