中隔子宫患者子宫内膜血管内皮生长因子受体的研究

目的 探讨子宫中隔患者中隔表面、子宫外壁内膜及正常非不孕不育女性子宫内膜中KDR或flt-1 mRNA量的差异. 方法 用RT-PCR检测增殖期38例子宫中隔患者外侧壁与中隔内膜组织及20例正常宫腔内膜KDR和flt-1mRNA量. 结果 外侧壁子宫内膜组织KDR及flt-1mRNA相对表达强度分别为2.51±1.12，1.22±0.51；中隔内膜组织相对表达强度分别为1.23±0.67, 0.71±0.31。而正常对照组子宫内膜组织KDR及flt-1mRNA相对表达强度分别为2.61±1.20,1.31±0.48。比较发现，中隔子宫外侧壁内膜组织中KDR和flt-1mRNA相对表达强度与正常对照组无显著性差异，而覆盖于中隔上的子宫内膜组织中两者的相对表达强度明显低于正常对照组（P＜0.05）；中隔子宫覆盖于中隔上的子宫内膜组织中KDR和flt-1mRNA相对表达强度明显低于外侧壁（P＜0.05）. 结论 子宫中隔患者覆盖于中隔的内膜组织中VEGF受体KDR和Flt- 1在mRNA水平上有一定程度的缺陷，当胚胎种植于中隔子宫的中隔上时并不影响胚胎着床，可产生不良的生殖结局

Differences of vascular endothelial growth factor receptor in the endometrium of septate uterus

0bjective To compare the messenger RNA (mRNA) expression of KDR and Flt-1 in the different endometrial locations of septate uterus and normal uterus in non-infertility women. Methods Proliferative endometrium tissues were obtained from the endometrium covering the septum and the endometrium lining the lateral wall of the uterus in thirty-eight.samples were obtained from patients with twenty normal uterus.By RT-PCR, amount of KDR and flt-1mRNA were detected in these endometrium tissues. Results Expression intensity of KDR and flt-1mRNA in endometrium lining the lateral wall of the uterus was 2.51 ± 1.12,1.22 ± 0.51， endometrium covering the septum were 1.23 ± 0.67, 0.71 ± 0.31 ，respectively；endometrium in non-infertility normal uterus were 2.61±1.20,1.31±0.48, respectively. The mRNA expression of KDR and Flt-1 was significantly lower in the endometrium lining the septum as compared with the endometrium covering the lateral wall of septate and normal uterus. Conversely, no differences were observed in mRNA expression of KDR and Flt-1 as compared with the endometrium covering the lateral wall of septate and normal uterus. Conclusions The results shown a local defect at the mRNA level of both KDR and Flt-1 in the septal area,which may help us to understand the clinical comportment and poor reproductive outcome of this müllerian anomaly in some cases.