羟苯磺酸钙对肾间质纤维化大鼠纤维连接蛋白表达的影响

目的研究羟苯磺酸钙对肾间质纤维化（renal interstitial fribrosis，RIF）大鼠纤维连接蛋白（fibronectin,FN）表达的影响。方法将42只SD大鼠随机分为假手术组（Sham组）、RIF模型组（UUO组）及羟苯磺酸钙治疗组（CDT组），采用单侧输尿管梗阻术制备RIF模型，分别观察术后7,14 d大鼠梗阻侧肾脏的纤维化程度和FN表达情况。结果与Sham组比较，UUO组大鼠术后7 d左肾肾间质纤维组织面积增加，FN表达增强，术后14 d加重（P＜0.01）。与UUO组比较，CDT组术后7和14 d左肾间质纤维组织面积均减少，FN表达均减弱（P＜0.01）。UUO组和CDT组大鼠左肾FN与RIF面积呈正相关（P＜0.01）。结论羟苯磺酸钙通过抑制FN的表达从而减轻UUO大鼠RIF。

Effect of Calcium Dobesilate on the Expression of Tubulointerstitial Fibronectin in Rats with Unilateral Ureteral Obstruction

Objective To assess the effects and mechanism of calcium dobesilate on the expression of fibronectin(FN) in the rat model of renal interstitial fibrosis(RIF) induced by unilateral ureteral obstruction(UUO). Methods Forty-two SD rats were randomly divided into the sham-operated group,UUO model group,calcium dobesilate treated group(CDT).The RIF model was established by ligating left ureters of the animals.The renal fibrosis and FN expression were checked after 7 and 14 d,respectively.and immunohistochemistry...