| 留兰香油对脂多糖引起的大鼠肺气肿样改变及对MMP-9表达的影响 |
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| 引用本文： | 刘君波,王砚,唐法娣,余晨曦,黄梦珊,赵小京,朱有法.留兰香油对脂多糖引起的大鼠肺气肿样改变及对MMP-9表达的影响[J].中国中药杂志,2011,36(8):1054-1059. |
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| 作者姓名： | 刘君波 王砚 唐法娣 余晨曦 黄梦珊 赵小京 朱有法 |
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| 作者单位： | 浙江大学,医学院,呼吸药物研究实验室,浙江,杭州,310058 |
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| 基金项目： | 浙江省科技计划项目(2008c23066) |
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| 摘 要： | 目的:观察留兰香油对脂多糖(LPS)所致大鼠肺气肿样改变及其对肿瘤坏死因子-α(TNF-α)、白细胞介素一1β(Ⅱ,1β)和组织抑制因子-1(TIMP-1)含量及基质金属蛋白酶-9(MMP-9)表达的影响.方法:反复气道内滴入脂多糖8周建立大鼠肺气肿样改变模型,设立正常对照组、模型组、地塞米松组及留兰香油10,30,100 mg·kg-1组,各给药组灌胃治疗4周.计数大鼠支气管肺泡灌洗液(BALF)中自细胞总数及其分类;肺组织病理切片行HE,AB-PAS染色,观察肺泡结构、气道炎症及杯状细胞化生,并对病理图像进行形态学测量分析;测定肺组织TNF-α,IL-1β和TIMP-1的含量及MMP-9的表达水平.结果:留兰香油10,30,100 mg·kg-1组及地塞米松组能明显减少LPS引起的肺泡破坏;留兰香油100 mg·kg-1组及地塞米松组能明显降低BALF中自细胞总数及分类中的中性粒细胞、淋巴细胞数量并抑制杯状细胞化生;地塞米松对TNF-α,IL-1β,TIMP-1及MMP-9的表达均有抑制作用;留兰香油30,100 mg·kg-1能分别降低肺组织匀浆中的TNF-α,IL-1β含量;留兰香油10,30,100 mg·kg-1对TIMP-1的表达无影响,却能显著降低肺组织中MMP-9的表达水平.结论:留兰香油对脂多糖引起的大鼠肺气肿样改变有一定的保护作用,其机制可能是留兰香油降低了肺组织TNF-α及IL-1β的含量,抑制了MMP-9的过表达.
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| 关 键 词： | 留兰香油 肺气肿样改变 脂多糖 基质金属蛋白酶-9 |
| 收稿时间： | 2010/6/23 0:00:00 |
Effect of spearmint oil on lipopolysaccharide induced emphysema-like changes and expression of matrix metalloproteinase-9 |
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| LIU Junbo,WANG Yan,TANG Fadi,YU Chenxi,HUANG Mengshan,ZHAO Xiaojing and ZHU Youfa.Effect of spearmint oil on lipopolysaccharide induced emphysema-like changes and expression of matrix metalloproteinase-9[J].China Journal of Chinese Materia Medica,2011,36(8):1054-1059. |
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| Authors: | LIU Junbo WANG Yan TANG Fadi YU Chenxi HUANG Mengshan ZHAO Xiaojing and ZHU Youfa |
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| Institution: | Zhejiang Respiratory Drugs Research Laboratory, College of Medicine, Zhejiang University, Hangzhou 310058, China;Zhejiang Respiratory Drugs Research Laboratory, College of Medicine, Zhejiang University, Hangzhou 310058, China;Zhejiang Respiratory Drugs Research Laboratory, College of Medicine, Zhejiang University, Hangzhou 310058, China;Zhejiang Respiratory Drugs Research Laboratory, College of Medicine, Zhejiang University, Hangzhou 310058, China;Zhejiang Respiratory Drugs Research Laboratory, College of Medicine, Zhejiang University, Hangzhou 310058, China;Zhejiang Respiratory Drugs Research Laboratory, College of Medicine, Zhejiang University, Hangzhou 310058, China;Zhejiang Respiratory Drugs Research Laboratory, College of Medicine, Zhejiang University, Hangzhou 310058, China |
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| Abstract: | |
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| Keywords: | spearmint oil emphysema-like changes lipopolysaccharide matrix metalloproteinase-9 |
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