凋亡相关基因Bcl-2、Bcl-Xl和BaX Mrna在大鼠缺血预处理脑组织中的表达

目的 检测脑缺血预处理大鼠脑组织中凋亡调控基因Ｂｃ１－２、Ｂｃ１－ｘ１和Ｂａｘ的表达。方法 假手术对照组、预处理对照组、预处理缺血组和缺血组Ｗｉｓｔａｒ大鼠分别再灌２４ｈ,４８ｈ和７２ｈ,采用半定量逆转录聚合酶链反应（ＲＴ－ＰＣＲ）和原位分子杂交（ＩＳＨ）方法对脑组织Ｂｃ１－２、Ｂｃ１－ｘ１和Ｂａｘ ｍＲＮＡ的表达进行检测。结果 半定量ＲＴ－ＰＣＲ显示：与假手术对照组相比,预处理对照组大鼠再灌２４

Expressions of apoptosis-associated genes Bcl-2, Bcl-xl and Bax mRNAs in rat cerebral ischemic preconditioning

Objective To determine the expressions of apoptosis-regulated genes Bcl-2,Bcl-xl and Bax in rat cerebral ischemic preconditioning.Methods Forty-eight Wistar rats were randomly divided into four groups:sham-operation control group(SHC group),cerebral ischemic preconditioning control group(CIPC group),ischemic preconditioning-cerebral ischemia group(IPCI group)and cerebral ischemic group(CI group).The rats were reperfused in 24h,48h or 72h in each group following ischemia.Semi-quantity RT-PCR and in situ hybridization were adopted to investigate Bcl-2,Bcl-xl and Bax mRNAs expressions.Results Using semi-quantity RT-PCR,compared with those in SHC group,Bcl-2 mRNA expression increased greatly after 24h and 48h reperfusions in CIPC group and after 24h reperfusion in IPCI,guoup(P<)005),and Bcl-xl mRNA expression rose obviously after 24h reperfusion in CIPC and IPCI groups,while Bax mRNA expression were not significantly different among four groups(WTBX]P>005)P>005).Using in situ hybridization,Bcl-2 and Bcl-xl mRNA expressions increased after 24h reperfusion in CIPC and IPCI group(P<005), without significant difference in Bax mRNA expression among four groups (P>005).Conclusions The expressions of Bcl-2 and Bcl-xl mRNAs increase following cerebral ischemic preconditioning,suggesting that anti-apoptosis genes play important roles in the maintenance of cellular viability after ischemia.