Studies on the human mixed lymphocyte culture. I. The influence of the irradiated autogenic and allogenic lymphocytes on DNA synthesis.

Fourteen patients with chronic renal failure and their prospective living donors were examined. DNA uptake by donors' monocultures, recipients' monocultures and mixed cultures was measured. The influence of X-ray treated cells on proliferative capacity of untreated cells in these three types of cultures was studied. The suppressing effect of irradiated cells was found in 52%, the enhancing one in 7% and no effect was observed in 41% of lymphocyte cultures. The possible explanations of the findings are discussed.