| 用比较基因组杂交技术(CGH)检测肾癌染色体畸变 |
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| 引用本文： | 王坚,徐仁方,宋青峰. 用比较基因组杂交技术(CGH)检测肾癌染色体畸变[J]. 基础医学与临床, 2010, 30(4): 398-400 |
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| 作者姓名： | 王坚 徐仁方 宋青峰 |
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| 作者单位： | 1. 解放军第303医院,泌尿外科,广西,南宁,530021
2. 常州市第一人民医院,泌尿外科,江苏,常州,213003 |
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| 摘 要： | 目的应用比较基因组杂交技术(CGH)分析原发性肾癌肿瘤组织中染色体异常变化,探讨肾癌细胞遗传物质的改变,揭示肾癌发生发展的内在本质及其与临床特征之间的关系。方法采用CGH技术对12例肾癌组织提取的全基因组DNA进行检测,以了解肾癌全基因组的变化。结果CGH技术检测的12例肾癌标本中均有染色体的畸变(扩增和/或缺失),常见的扩增区是1p、4p、5q、7p、9p、16p,常见的缺失区是3q、4q、6q、9q、14q、18q。结论原发性肾癌存在广泛的遗传物质不平衡现象,肾癌细胞染色体扩增和/或缺失可能是肾癌发生发展的基础。
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| 关 键 词： | 肾癌 比较基因组杂交 染色体 |
| 收稿时间： | 2009-06-29 |
| 修稿时间： | 2009-10-27 |
Comparative genomic hybridization technique detects the chromosomal aberration in renal carcinoma |
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| WANG Jian,XU Ren-fang,SONG Qing-feng. Comparative genomic hybridization technique detects the chromosomal aberration in renal carcinoma[J]. Basic Medical Sciences and Clinics, 2010, 30(4): 398-400 |
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| Authors: | WANG Jian XU Ren-fang SONG Qing-feng |
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| Affiliation: | 1.Dept.of Urology;the 303th Hospital of People's Liberation Army;Nanning 530021;2.Dept.of Urology;the First People'sHospital of Changzhou;Changzhou 213003;China |
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| Abstract: | Objective To detect the chromosomal aberration in the cells of renal carcinoma and to evaluate the relationship between the chromosomal aberration and development of renal carcinoma.Methods CGH technology was used to analyze the global genomic aberration in cancer cells from 12 patients with renal carcinoma.Results All of 12 cases deceted by CGH showed chromosomal aberrations.The common extension regions of renal carcinoma were 1p,4p,5q,7p,9p and 16p.The common deletion regions of renal carcinoma were 3q,4q... |
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| Keywords: | renal carcinoma comparative genomic hybridization chromosome |
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