姜黄素对脂多糖诱导大鼠急性肺损伤的保护作用

目的观察姜黄素对脂多糖诱导大鼠急性肺损伤（ALI）的保护作用。方法 Wistar大鼠随机分为6组：空白组、模型组、地塞米松组和姜黄素三个不同剂量组（50、100、200mg.kg-1）。LPS气管滴注复制ALI模型,6h后收集支气管肺泡灌洗液（BALF）,检测TNF-α、IL-1β浓度及蛋白含量;称重法检测肺组织湿/干重比和肺含水量。结果姜黄素剂量依赖性降低LPS所致ALI模型BALF中TNF-α、IL-1β及蛋白量增加,降低肺湿/干重比、肺含水量。结论姜黄素对LPS所致ALI有保护作用,其可能的机制为调节炎症介质的产生有关。

Effect of Curcumin on Acute Lung Injury Induced by Lipopolysaccharide in Rats

Objective To observe the protective effect of curcumin on acute lung injury（ALI）induced by Lipopolysaccharides（LPS）in rats.Methods Wistar rats were randomly divided into 6 groups：control,model,dexamethasone and curcumin groups（50,100,and 200 mg·kg-1）.The model of ALI was established by intratracheal instillation of LPS.The TNF-α,IL-1β and protein levels in bronchoalveolar lavage fluid（BALF）were detected.In addition,the ratio of lung wet weight to dry weight（W/D）and water content were also measured.Results Curcumin could significantly decrease the levels of TNF-α,IL-1β,protein in BALF,the W/D ratio and water content in a dose-dependent manner.Conclusion Curcumin has the protective effect againt ALI of rats induced by LPS through modulating the release of inflammatory mediators.