氯胺酮对新生大鼠海马乙酰胆碱酯酶和乙酰胆碱转移酶的影响

目的探讨氯胺酮对新生大鼠学习记忆和海马乙酰胆碱酯酶(AchE)、乙酰胆碱转移酶(ChAT)的影响。方法 80只7 d龄大鼠随机分为C、K1、K2、K3组,每组20只。C组给予生理盐水2 ml;K1组单次腹腔注射氯胺酮80 mg/kg,K2、K3组分别按同等剂量注射2、3 d。末次给药后1 d各组处死10只、余大鼠至21 d龄行Morris水迷宫实验。迷宫实验后检测海马AchE和ChAT水平。结果水迷宫结果:C组与K1组比较、K2组与K3组比较差异均无统计学意义;K2、K3组较C、K1组潜伏期延长且穿越平台次数减少(P<0.05)。酶测定:8 d龄,各组间差异有统计学意义(P<0.05)。27 d龄,C组与K1组比较、K2组与K3组比较差异均无统计学意义,K2、K3组较C、K1组差异有统计学意义(P<0.05)。结论氯胺酮引起新生大鼠空间辨别记忆能力下降,可能与海马胆碱能系统有关;单次给药能引起短期内海马胆碱能系统变化,而重复给药则会导致较长时间的影响。

Effect of ketamine anesthesia on AchE and ChAT of hippocampus in neonatal rats

Objective To investigate the effect of ketamine on the capabilities of memory,content of and learning and AchE,ChAT of hippocampus in neonatal rats.Methods 80 SD rats,aged 7 days were randomly divided into group C,K1,K2,K3,20 rats in each group.Group C was treated with 2 ml physiological saline;group K1 was treated with ketamine 80 mg/kg once;group K2 was treated with ketamine gomgllcg once a day for 2 days;group K3 was treated with ketamine gomgllcg once a day for 3 days.10 rats were killed each group the next day after the last injection.After testing the behavior at the 21st day by Morris water maze(MWM),the rest of rats were killed and isolated the hippocampus for determination of ChAT and AchE.Results MWM: There was no significant difference between group C and group K1,group K2 and group K3,the latency period way longer and the frequency to cross the plat way less in group K2,K3 than that in group C,K1(P<0.05).The results of enzymes:8 days age,there were significant difference(P<0.05);27 days age,there was no significant difference between group C and group K1,group K2 and group K3.There was significant difference between group K1,C and group K2,K3(P<0.05).Conclusion Ketamine can decrease the spatial learning and memory behavior of neonatal rats,and it may be related to the cholinergic nervous system of hippocampus;single done causes short-term change,and repeated medicine can lead to long-time influence.