高效液相色谱法测定人血浆中左布比卡因的浓度

目的建立高效液相色谱法测定人血浆中左布比卡因的浓度。方法以罗哌卡因为内标,色谱柱为Hypersil C_(18)柱(200 mm×4.6 mm,5μm),流动相采用0.01 mol·L~(-1)磷酸二氢钾-乙腈(87:13,V:V,pH=3.4),流速2.0 mL·min~(-1),检测波长为210 nm,柱温40℃。结果标准曲线方程为Y=0.310 1 X+ 0.008 9,r=0.999 7,左布比卡因的质量浓度在0.012 5～2 mg·L~(-1)范围内呈良好的线性关系,最低检测限为0.01 mg·L~(-1);方法回收率在96%～105%之间;日间、日内精密度RSD均<5%。结论本方法可用于临床上左布比卡因血药浓度的监测和药动学研究。

Determination of levobupivacaine in human plasma by high-performance liquid chromatography

AIM To develop a high performance liquid chromatography (HPLC) method for the determi- nation of levobupivacaine in human plasma.METHODS Using ropivacaine as internal standard,the sample was introduced into a LC-6A HPLC system equipped with the column of Hypersil C_(18) (200 mm×4.6 mm,5μm).The mobile phase was composed of potassium dihydrogen phosphate (0.01 mol·L~(-1)) and acetonitrile (87 :13,V:V,pH=3.4).The flow rate was 2.0 mL·min~(-1),the detective wavelength was 210 nm and the temperature of the column was 40℃.RESULTS The standard curve was Y=0.3101X+0.0089 (r=0.9997). The linear range was 0.012 5-2 mg·L~(-1) and the limit of detection was 0.01 mg·L~(-1).The average recovery was 96 %-105 %.The RSD of inter-day and intra-day were all less than 5 %.CONCLUSION The HPLC method is suitable for monitoring levobupivacaine concentration in clinical plasma samples and for pharmacokinetics study.