胃癌组织中KAI1、nm23及P53的表达及其临床意义

目的:探讨正常胃黏膜、不典型增生胃黏膜及癌组织中KAI1、nm23及P53蛋白的表达．方法:应用SP法免疫组化检测22例正常胃黏膜,65例不典型增生胃黏膜及74N胃癌组织中的KAI1、nm23及P53蛋白的表达．结果:正常胃黏膜、不典型增生胃黏膜及胃癌组织中,KAI1和nm23阳性率呈降低趋势,组间差异性有统计学意义(x2=20．885, P<0．001;x2=29．133,P<0．05):P53蛋白阳性表达率呈增加趋势,组间差异性有统计学意义(x2=21．954,P<0．001)．Fisher精确概率检验显示:在胃癌组中不同的浸润深度、有无淋巴结转移和脉管侵犯组内KAI1、nm23及 P53组阳性表达率的差异性有统计学意义(x2 =20．885,P<0．001;x2=29．133,P<0．05;x2= 21．954,P<0．001);而在年龄、性别组间的差异性无统计学意义．Spearman等级相关分析显示 KAI1与nm23表达呈正相关(r=0．859,P<0．05); KAI1与P53表达呈负相关(r=-0．859,P<0．05), nm23与P53表达呈负相关(r=-0．874,P<0．05) 结论:抑癌基因KAI1与nm23的缺失以及P53 蛋白的过表达可能是胃癌发生、发展及浸润和转移的重要原因之一．

Expression of KAI1, nm23 and P53 in gastric carcinoma and their clinical significances

AIM: To investigate the expression of KAI1, nm23 and P53 protein as well as their significances in the development of gastric carcinoma. METHODS: The expression of KAI1, nm23 and P53 protein were detected by SP immunohis-tochemistry in gastric atypical hyperplasia (n = 65), gastric carcinoma (n = 74) and normal gastric tissues (n = 22). RESULTS: The positive rates of KAI1 and nm23 protein expression were decreased with the malignancy of gastric tissues, and there were significantly differences between normal gastric tissues, atypical hyperplasia and carcinoma (KAI1:x2 = 20.885, P < 0.001; nm23:x2 = 29.133, P < 0.05), while the positive rate of P53 protein expression was in the opposite tendency (x2 = 21.954, P < 0.001). The expression of KAI1, nm23 and P53 protein were notably correlated with the depth of invasion, lymph nodes metastasis and vessel invasion (x2 = 20.885, P < 0.001; x2 = 29.133, P < 0.05; x2 = 21.954, P < 0.001), but they were not correlated with the age and gender of patients. Besides, the expression of KAI1 was positively related to that of nm23 (r = 0.859, P < 0.05), but negatively related to that of P53 (r = -0.859, P < 0.05). Negative correlation was also found between the expression of nm23 and P53 (r = -0.874, P < 0.05). CONCLUSION: Loss expression of KAI1 and nm23 and over-expression of P53 may play important roles in the progression and lymph node metastasis of gastric carcinoma.