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Expression of FK506-binding protein 52 (FKBP52) in chorionic villi with early recurrent spontaneous abortion |
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| Authors: | Hong-Yan Chen Ou-Yang Li Hong Xu Xiao-Jing Fan Hai-Feng Liang |
| Affiliation: | 1. Division of Maternal-Fetal Medicine, Prenatal Diagnosis Center, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China and;2. Department of Obstetrics and Gynecology, the First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China |
| Abstract: | Objective: To investigate the mRNA and protein expression of FK506-binding protein 52 (FKBP52) in the chorionic villi of patients with recurrent spontaneous abortion (RSA) and normal women during early pregnancy. Methods: Fresh chorionic villus tissues were collected from 60 subjects. A total of 30 patients with a history of RSA were enrolled into the RSA group and 30 normal pregnant women were enrolled into the control group. The FKBP52 mRNA expression levels in chorionic villi of the RSA patients and healthy controls were measured via semiquantitative RT-PCR. The protein distribution and expression levels of FKBP52 in chorionic villi were analyzed through immunohistochemistry (IHC). The correlation between FKBP52 expression and RSA was analyzed. Results: We demonstrated that FKBP52 mRNA is expressed in chorionic villi samples of normal pregnancy and RSA. RSA patients exhibited significantly lower FKBP52 gene expression levels compared with those in normal pregnancies (p?p? Conclusions: FKBP52 protein levels were decreased in the chorionic villi of RSA patients, which indicate that the decrease in FKBP52 may be associated with RSA. The low FKBP52 mRNA expression level, which is consistent with the IHC result, may affect embryonic development and even lead to abortion. FKBP52 may be involved in the pathogenesis of RSA and new therapies that increase the FKBP52 expression may help treat RSA. |
| Keywords: | Chorionic villi FKBP52 immunohistochemistry recurrent spontaneous abortion RT-PCR |