Cyclin D1、p16及Rb在视网膜母细胞瘤中的表达及其相关性

目的探讨CyclinD1、p16、Rb在视网膜母细胞瘤（retinoblastoma，RB）中的表达及其生物学意义。方法采用免疫组织化学方法（SP法）检测CyclinD1、p16及Rb在46例常规石蜡包埋的视网膜母细胞瘤标本中的表达，并在生物学行为及分化程度不同的视网膜母细胞瘤之间进行比较。结果正常视网膜组织与视网膜母细胞瘤组织中CyclinD1、p16及Rb蛋白表达差异有极显著性意义（P〈0．01）；视网膜母细胞瘤中，Rb与p16、CyclinD1表达之间无明显相关性（P〉O．05）；p16表达率在生物学行为不同的视网膜母细胞瘤之间差异显著，30例视神经未浸润组视网膜母细胞瘤的p16阳性表达率明显高于16例视神经浸润组（P〈0．05）；Rb及p16蛋白表达阳性率随组织学分级降低而下降，分化组与未分化组视网膜母细胞瘤中Rb及p16蛋白表达率差异有极显著性意义（P〈0．01）。结论p16、Rb、CyclinD1在视网膜母细胞瘤发生发展中起着重要作用。CyclinD1的过表达与p16和Rb的表达缺失或失活是视网膜母细胞瘤发生的重要分子事件，p16蛋白失表达可能与其视神经浸润能力有关，Rb及p16蛋白失表达则与其分化程度相关。

Expression of Cyclin D1,p16 and Rb in Retinoblastoma and Their Relationship

Objective To find out the expression and their biological significance of Cyclin D1,p16 and Rb in retinoblastoma(RB).Methods The expression of Cyclin D1,p16 and Rb proteins in routinely paraffin-embedded sections from 46 cases of RB was detected by using SP immunohistochemical method,and the expressive discrepancy was compared among RB cases having different biological behaviors.Results There was significant difference in the expression intensity of Cyclin D1,p16 and Rb proteins between normal retina tissue and RB(P<0.01).There was no obvious relationship between the expression of Rb and p16,Cyclin D1 in RB(P>0.05).There was significant difference in the p16 expression among RB cases having different biological behaviors: The positive expression rate of p16 in the 30 RB cases without infiltration was significantly higher than that in the 16 RB cases with infiltration(P<0.05);The positive expression rate of Rb and p16 proteins was decreased with the deterioration of histological grades,and the significant differences in the positive expression rate of Rb and p16 proteins were found between the differentiation group and un-differentiation group(P<0.01).Conclusion Abnormal expression of p16,Rb and Cyclin D1 in RB may play an important role in the occurrence and development of RB.The overexpression of Cyclin D1 and the deletion or inactivation of the p16 and Rb expression is the important molecule event in the occurrence of RB;The loss of the expression of p16 protein may be related with the invasion ability in the optic nerve,while the loss of the expression of Rb and p16 proteins related with differentiation degree of RB.