地黄生长期根叶中梓醇及总环烯醚萜苷含量变化

地黄主要活性成分为环烯醚萜苷，其中含量最高的是梓醇。该文主要对地黄生长发育过程中根叶所含总环烯醚萜苷和梓醇的含量变化进行研究。以地黄85-5为材料，于地黄不同生长期进行不同直径块根、不同叶龄的样品采集，分别采用紫外分光光度法和高效液相色谱法测定总环烯醚萜苷和梓醇的含量。研究结果表明，7—9月同一生长期地黄不同叶龄中总环烯醚萜苷和梓醇含量，幼嫩组织中含量较高，随着叶龄的增加而逐步下降；比较各生长期相同着生部位的叶片中梓醇和总环烯醚萜苷含量，大多数部位的叶片在10月达到最高；同一生长期块根直径越大，有效成分含量越高；梓醇含量同总环烯醚萜苷含量呈正相关。以期通过以上初步结论可以为地黄的萜类代谢调控研究和栽培技术提供参考依据。

Study on distribution and dynamic accumulation of catalpol and total iridoid in fresh Rehmannia glutinosa

Iridoid glycosides were the main active ingredient of Rehmannia glutinosa, of which catalpol has the highest content. This work will provide theoretical basis for metabolic study and cultivation of iridoids on the basis of the dynamic accumulation of catalpol and total iridoids in the growth of R. glutinosa. The samples of rehmannia 85-5 were gathered in the same filed from July to October. The contents of catalpol and total iridoid glycosides were measured by HPLC and specteophotometric, respectively. The results showed that youngest leaves had the higher content of catalpol and total iridoid glyosides than that of the other two leaf ages in the same growth stage from July to September, while their content of catalpol and total iridoid glycosides were all decreased as the growth of leaves of R. glutinosa. The content of catalpol didn't differ significantly from July to September, whereas it has significantly increased in October in the three leaf stage. In the same stage, the wider the root diameter is, the higher content of the effective components are. In August and September, the total iridoid glycosides have the fastest accumulation. The content of catalpol was increased as the accumulation of total iridoid glycosides.