| 耳聋左慈丸及有效拆方拮抗庆大霉素诱导毛细胞凋亡的实验研究 |
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| 引用本文： | 王静,郭春荣,董杨,金国琴,郭瑞新,韩志芬,蔡西晨,施建蓉. 耳聋左慈丸及有效拆方拮抗庆大霉素诱导毛细胞凋亡的实验研究[J]. 中国中药杂志, 2010, 35(18): 2464-2468 |
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| 作者姓名： | 王静 郭春荣 董杨 金国琴 郭瑞新 韩志芬 蔡西晨 施建蓉 |
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| 作者单位： | 1. 上海中医药大学,教学实验中心,上海,201203
2. 上海中医药大学,生化教研室,上海,201203 |
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| 基金项目： | 上海市科委基础研究重点科技项目(09JC1413500);上海市教委重点学科项目(J50301) |
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| 摘 要： | 目的:探讨耳聋左慈丸及其有效拆方(熟地黄泽泻药对、泽泻单味药)对庆大霉素诱导内耳听毛细胞凋亡相关蛋白及酶活性变化的影响。方法:复制内耳螺旋器体外培养庆大霉素损伤模型,采用原位荧光标记及免疫荧光标记观察庆大霉素损伤毛细胞区域凋亡酶Caspases活性、凋亡相关蛋白Bcl-2和Bax蛋白表达量的改变及中药的影响。结果:与正常对照组比较,0.3mmol.L-1庆大霉素能够引起内耳毛细胞Caspase-3,Caspase-9酶活性明显增强,凋亡调节因子Bcl-2/Bax蛋白表达明显下降。与模型组比较,耳聋左慈丸对Caspase-9酶活性有显著抑制作用,对Caspase-3酶活性有抑制趋势,对Bcl-2/Bax蛋白表达有明显上调作用;熟地黄泽泻药对、泽泻单味均能明显抑制Caspase-3,Caspase-9酶活性,显著上调Bcl-2/Bax蛋白表达。结论:耳聋左慈丸、熟地黄泽泻药对及泽泻单味可能通过纠正线粒体凋亡信号转导途径的异常变化,拮抗庆大霉素对毛细胞的损伤。
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| 关 键 词： | 细胞凋亡 耳聋左慈丸 庆大霉素 耳毒性 毛细胞 |
| 收稿时间： | 2010-02-07 |
Effects of Erlong Zuoci pills and its effective disassembled prescriptions on gentamycin induced hair cell apoptosis |
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| WANG Jing,GUO Chunrong,DONG Yang,JIN Guoqin,GUO Ruixin,HAN Zhifen,CAI Xichen and SHI Jianrong. Effects of Erlong Zuoci pills and its effective disassembled prescriptions on gentamycin induced hair cell apoptosis[J]. China Journal of Chinese Materia Medica, 2010, 35(18): 2464-2468 |
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| Authors: | WANG Jing GUO Chunrong DONG Yang JIN Guoqin GUO Ruixin HAN Zhifen CAI Xichen SHI Jianrong |
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| Affiliation: | Department of Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Biochemistry, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Department of Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| Abstract: | |
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| Keywords: | apoptosis Erlong Zuoci pills gentamycin ototoxicity hair cell |
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