| HPLC法测定脂质体中足叶乙苷的含量研究 |
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| 引用本文： | 时军,程怡,陈伟鸿. HPLC法测定脂质体中足叶乙苷的含量研究[J]. 安徽医药, 2010, 14(5): 544-545. DOI: 10.3969/j.issn.1009-6469.2010.05.022 |
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| 作者姓名： | 时军 程怡 陈伟鸿 |
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| 作者单位： | 广东药学院中药学院;广州中医药大学中药学院,广东,广州,510006 |
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| 基金项目： | 高等学校博士学科点专项科研基金 |
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| 摘 要： | 目的建立足叶乙苷脂质体的含量测定方法。方法采用HPLC法,以Phenomenex Synergi C18柱（4.6 mm×250μm,5μm）为色谱柱;甲醇-0.2%NaAc-HAc缓冲液为流动相;流速：1.0 ml.min-1,检测波长：285 nm,进样量：20μl。结果足叶乙苷线性范围为10.5~210 mg.L-1,加样回收率为99.90%;精密度符合要求。结论HPLC测定法稳定可靠,可用于足叶乙苷脂质体中的药物含量测定方法。
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| 关 键 词： | HPLC 足叶乙苷 脂质体 |
Determination of etoposide content in liposomes by HPLC |
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| SHI Jun,CHENG Yi,CHEN Wei-hong. Determination of etoposide content in liposomes by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2010, 14(5): 544-545. DOI: 10.3969/j.issn.1009-6469.2010.05.022 |
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| Authors: | SHI Jun CHENG Yi CHEN Wei-hong |
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| Affiliation: | SHI Jun1,CHENG Yi2,CHEN Wei-hong2(1.Guangdong Pharmaceutical University;2.Guangzhou University of Chiese Medicine,Guangzhou,Guangdong 510006,China) |
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| Abstract: | Aim To develop an HPLC assay for the determination of etoposide in liposomes.Methods The sample was analyzed on a Phenomenex Synergi C18 column by using methanol-water-0.2%NaAc as mobile phase.The detection wavelength was at 285 nm with a flow rate of 1.0 ml·min-1.Results The linear range was from 10.5 to 210 mg·L-1.The recovery ratio was 99.90% and precision fits the requirements.Conclusions HPLC method can be suitable for determination of etoposide liposomes. |
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| Keywords: | HPLC |
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