Purification of 45 KDa estramustine binding protein (EMBP) and preparation of its antibody |
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| Authors: | H Yuasa H Yamanaka K Imai T Mashimo H Kohno |
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| Affiliation: | Department of Urology, Gunma University School of Medicine. |
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| Abstract: | ![]()
The purification of 45 KDa EMBP and the production of monospecific anti-serum is described. 45 KDa EMBP was purified by relatively simple methods using ion exchange HPLC (TSK-GEL DEAE-5PW column) and size exclusion HPLC (TSK-GEL G3000SW column). The results clearly demonstrated the speed and simplicity of the method using these columns, compared to previously-described methods for purification of 45 KDa EMBP. |
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