| 显微切割鼻咽癌组织16号染色体杂合性缺失的研究 |
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| 引用本文： | 郭颖,方嬿,梁启万,李辉梅,郝亚萍,曾益新.显微切割鼻咽癌组织16号染色体杂合性缺失的研究[J].癌症,2000,19(1):1-5. |
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| 作者姓名： | 郭颖 方嬿 梁启万 李辉梅 郝亚萍 曾益新 |
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| 作者单位： | 中山医科大学肿瘤防治中心肿瘤研究所,广东广州510060 |
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| 基金项目： | 国家自然科学基金,美国CMB基金,中国科学院资助项目,广东省博士启动基金,国家"211"工程建设项目,39825511,,,,,,,,, |
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| 摘 要： | 目的：研究鼻咽癌中１６号染色体的缺失情况。方法：采用显微切割的方法获取肿瘤组织,然后用ＰＣＲ的方法以１６号染色体上的８对引物对３８例鼻咽癌进行分析。结果：３８例鼻咽癌组织中所有标本至少有一个位点出现有杂合笥缺失,其中Ｄ１６Ｓ５３３出现杂合性缺失的频率最高,占８６．１％（３１／３３）,此外,杂合性缺失频率超过５０％的有引物Ｄ１６Ｓ３９８、Ｄ１６Ｓ３００和Ｄ１６Ｓ４２０。分别占７８．８％（２６／３３）
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| 关 键 词： | 鼻咽肿瘤 16号染色体 杂合性缺失 |
| 文章编号： | 1000－467X(2000)01－0001－05 |
| 修稿时间： | 1999－10－12 |
Loss of heterozygosity analysis of micro-dissected nasopharyngeal carcinoma tissues at chromosome 16 |
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| GUO Ying,FANG Yan,LIANG Qi-wan,et al..Loss of heterozygosity analysis of micro-dissected nasopharyngeal carcinoma tissues at chromosome 16[J].Chinese Journal of Cancer,2000,19(1):1-5. |
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| Authors: | GUO Ying FANG Yan LIANG Qi-wan |
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| Institution: | GUO Ying,FANG Yan,LIANG Qi-wan,et al. Department of Etiology,Cancer Center,Sun Yat-sen University of Medical Sciences,Guangzhou 510060,P. R. China |
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| Abstract: | Objective: To analyze the loss of heterozygosity (LOH) of chromosome 16 in nasopharyngeal carcinoma (NPC). Methods: Tumor tissues were obtained with microdissetion.PCR was used to analyse 38 NPC samples using eight primers on chromosome 16. Results: All of 38 cases were showed with LOH in at least one of the loci analyzed.Among these primers, D16S533 had the highest frequency of LOH, which was 86.1 % (31/33). High frequent LOH (> 50% ) occured at three other loci including D16S398, D16S390 and D16S420, the presentages were 78.8% (26/33 ). 69.4% (25/33 ) and 57.6 (19/33 ). respectively. Conclusions: From this investigation, we found high frequent LOH occurred at chromosome 16p 12. 3, 16q12. 2 and 16q21-22.1I for the first time. These results indicate that more than one (TSGs) related to NPC may harbored at these regions. |
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| Keywords: | Nasopharyngeal neoplasm Chromosome 16 LOH |
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