神经酰胺在高糖诱导的人脐静脉内皮细胞凋亡中的作用

目的 观察神经酰胺（CER）在高糖诱导的人脐静脉内皮细胞凋亡中的作用。方法分别以流式细胞术和TUNEL法、高压液相色谱法等检测细胞凋亡、CER和酸性鞘磷酯酶（ASM）。结果（1）高糖呈浓度依赖性引起细胞内CER和酸性ASM活性增加，左旋葡萄糖无类似效应；（2）地昔帕明可抑制高糖诱导的内皮细胞凋亡，外源性（CER）可逆转其抑制作用；（3）外源性CER及酸性ASM可诱导内皮细胞凋亡；（4）高糖诱导的细胞内CER增加与高糖浓度及细胞凋亡均正相关。结论由酸性ASM水解引起的细胞内神经酰胺增加参与了高糖诱导的内皮细胞凋亡。

The role of ceramide in the high-D-glucose-induced apoptosis in human umbilical vein endothelial cells

Objective To explore the role of ceramide（CER） in the high-Dglucose-induced apoptosis in human umbilical vein endothelial cells （HUVECs）. Methods Apoptosis,CER and acidic sphingomyelinase（A Smase） were detected by TUNEL method/flow cytometry analysis （apoptosis）, LC/MS/MS （CER）, HPLC（ A- Smase） respectively. Results ①High-D-glucose,but not L-glucose, caused the concentration-dependent increases of intraeellular CER and A-SMase activity in HUVECs. But desipramine（DES） could inhibit the above effects of Dglucose（P〈0. 05）. ②DES inhibited the high-D-glucose-induced apoptosis in HUVECs, and exogenous CER could reverse the inhibition of DES（P〈0. 05）. ③Exogenous CER and A-Srnase increased the apoptosis in HUVECs（P〈0. 05）. ④The positive correlation of high-D-glucose-induced intra-cellular CER with D-glucose concentration and apoptosis was fotmd（P=0. 000）. Conclusions The high-D-glucose-induced apoptosis in HUVECs takes place through the acidic SMase-ceramide signal pathway.